VEGF, VEGFRs expressions and activated STATs in ovarian epithelial carcinoma
VEGF, VEGFRs expressions and activated STATs in ovarian epithelial carcinoma
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DOI：
10.1016/j.ygyno.2004.05.056
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发表时间：
2004-09-01
影响因子：
4.7
通讯作者：
Lu, WG
中科院分区：
文献类型：
--
作者：
Chen, HZ;Ye, DF;Lu, WG
客观的。探讨血管内皮生长因子(VEGF)、VEGFRs及信号转导和转录激活因子(STATs)在卵巢上皮癌组织中的表达及其相互关系。方法。采用免疫组织化学染色法检测42例原发性卵巢上皮癌、29例卵巢良性肿瘤和11例正常卵巢组织标本中VEGF、VEGFR1、VEGFR2、P-STAT1、P-STAT3、P-STAT5、P-STAT6蛋白的表达情况。卵巢癌中的VEGF显着高于良性和正常卵巢组织。 VEGFR 表达与 VEGF 表达一致。卵巢癌肿瘤细胞和内皮细胞中P-STAT3和P-STAT5的表达显着高于良性和正常卵巢组织。在内皮细胞中，VEGFR1和P-STAT5的表达彼此密切相关，VEGFR2和P-STAT3的表达也密切相关。然而，在卵巢癌细胞中，VEGF、VEGFR1和VEGFR2的表达与P-STAT3和P-STAT5显着相关，但与P-STAT1和P-STAT6不相关。结论。存在VEGF、VEGFRs的过表达以及STAT3、STAT5的激活。此外，这些结果表明卵巢癌细胞分泌的VEGF可能通过卵巢癌本身中的VEGFR激活STAT通路。 (C) 2004 Elsevier Inc. 保留所有权利。
Objective. To investigate the expressions of vascular endothelial growth factor (VEGF), VEGFRs and activation of signal transducers and activators of transcription (STATs) in ovarian epithelial carcinoma and the relationships among them.Methods. The tissue samples of 42 primary ovarian epithelial carcinoma, 29 benign ovarian tumor and 11 normal ovarian tissue were used to determine the expression of VEGF, VEGFR1, VEGFR2, P-STAT1, P-STAT3, P-STAT5 and P-STAT6 proteins by inummohistochemical staining.Results. VEGF in ovarian carcinomas was significantly higher than that in benign and normal ovarian tissues. VEGFRs expression was in agreement with VEGF expression. In tumor cells and endothelial cells of ovarian carcinomas, expressions of P-STAT3 and P-STAT5 were significantly higher than those in benign and normal ovarian tissues. In endothelial cells, the expression of VEGFR1 and P-STAT5 closely correlated with each other, as well as VEGFR2 and P-STAT3. However, in ovarian carcinoma cells, expressions of VEGF, VEGFR1 and VEGFR2 were significantly correlated with P-STAT3 and P-STAT5, but not with P-STAT1 and P-STAT6.Conclusions. There exist overexpressions of VEGF, VEGFRs, and STAT3, STAT5 activation. Furthermore, these results indicate that VEGF secreted by ovarian carcinoma cells may activate STAT pathway via VEGFRs in ovarian carcinoma themselves. (C) 2004 Elsevier Inc. All rights reserved.