Demonstration of Persistent Infections and Genome Stability by Whole-Genome Sequencing of Repeat-Positive, Same-Serovar Chlamydia trachomatis Collected From the Female Genital Tract.
Demonstration of Persistent Infections and Genome Stability by Whole-Genome Sequencing of Repeat-Positive, Same-Serovar Chlamydia trachomatis Collected From the Female Genital Tract.
复制标题
通过对从女性生殖道收集的重复阳性、相同血清型沙眼衣原体进行全基因组测序，证明持续感染和基因组稳定性。
DOI：
10.1093/infdis/jix155
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Rockey,DanielD
中科院分区：
文献类型：
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作者：
Suchland,RobertJ;Dimond,ZoeE;Putman,TimothyE;Rockey,DanielD
背景沙眼衣原体对人类反复或长期感染的生物学研究甚少。由于反复或持续感染与人类严重的并发症相关，了解这些过程可能会改善临床管理和公共卫生疾病控制。沙眼衣原体是从先前描述的一组患者中收集的，在该组患者中，个人被证明在很长一段时间内感染了单一血清型。结果来自7名患者中的5名患者的数据显示，令人信服的证据表明，这些患者能够携带相同的菌株3-5年。这些菌株中的突变是累积的，非常罕见，并且与任何单一的蛋白质或途径无关。从1名相隔3年的患者身上分离的J血清型菌株在整个基因组中没有积累单一的碱基变化。相比之下，两名患者的序列结果显示，在1-5年内，每个患者只感染Ia血清型菌株，发现了多个相同的血清型感染。结论这些数据证明了患者在面对反复抗生素治疗时长期持续的例子，表明病原体突变策略在患者中持续这种病原体并不重要。
BackgroundThe biology of recurrent or long-term infections of humans byChlamydia trachomatisis poorly understood. Because repeated or persistent infections are correlated with serious complications in humans, understanding these processes may improve clinical management and public health disease control.MethodsWe conducted whole-genome sequence analysis onC. trachomatisisolates collected from a previously described patient set in which individuals were shown to be infected with a single serovar over a lengthy period.ResultsData from 5 of 7 patients showed compelling evidence for the ability of these patients to harbor the same strain for 3–5 years. Mutations in these strains were cumulative, very uncommon, and not linked to any single protein or pathway. Serovar J strains isolated from 1 patient 3 years apart did not accumulate a single base change across the genome. In contrast, the sequence results of 2 patients, each infected only with serovar Ia strains, revealed multiple same-serovar infections over 1–5 years.ConclusionsThese data demonstrate examples of long-term persistence in patients in the face of repeated antibiotic therapy and show that pathogen mutational strategies are not important in persistence of this pathogen in patients.