Neuronal excitability and network formation on optically transparent electrode materials.
Neuronal excitability and network formation on optically transparent electrode materials.
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DOI：
10.1109/ner.2017.8008315
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发表时间：
2017-05
期刊：
影响因子：
--
通讯作者：
Purcell EK
中科院分区：
文献类型：
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作者：
Thompson CH;Khan SA;Khan WA;Li W;Purcell EK
随着基因编码的光学工具来触发或报告神经元活动的出现，用于神经接口的多电极阵列（MEA）的新设计结合了刺激或记录神经活动的光学和电学模式。同样地，对改善植入MEA的生物相容性的需求已经将该领域转向在装置制造中使用更软、更顺应的材料。然而，关于多边环境协定中使用的材料对连接的单个神经元和神经元网络功能的影响，现有信息有限。我们评估了大鼠皮层神经元对通常用于构建“下一代”设备的光学透明材料的反应：氧化铟锡（ITO），聚对二甲苯-C和聚二甲基硅氧烷（PDMS）。我们发现，在ITO上培养的神经元中，神经元网络的形成和对电刺激的尖峰响应增强。我们观察到PDMS上培养的神经元之间的兴奋性和突触连接性降低。我们假设ITO的上级导电性和PDMS的次优神经元附着有助于我们的结果。
With the advent of genetically-encoded optical tools to trigger or report neuronal activity, new designs for multielectrode arrays (MEAs) used in neural interfacing incorporate both optical and electrical modes of stimulating or recording neural activity. Likewise, the need to improve upon the biocompatibility of implanted MEAs has moved the field towards the use of softer, more compliant materials in device fabrication. However, there is limited available information on the impact of the materials used in MEAs on the function of interfaced individual neurons and neuronal networks. We assessed the responses of rat cortical neurons on optically transparent materials commonly used in the construction of “next-generation” devices: indium tin oxide (ITO), parylene-C, and polydimethylsiloxane (PDMS). We found that neuronal network formation and spiking responses to electrical stimulation were enhanced in neurons cultured on ITO. We observed reduced excitability and synaptic connectivity between neurons cultured on PDMS. We hypothesize that the superior conductivity of ITO and suboptimal neuronal attachment to PDMS contributed to our results.