The effect of temperature fluctuations on the cytoskeletal organisation and chromosomal constitution of the human oocyte
The effect of temperature fluctuations on the cytoskeletal organisation and chromosomal constitution of the human oocyte
复制标题
DOI：
10.1017/s0967199400002793
复制
发表时间：
1995-11-01
期刊：
影响因子：
1.7
通讯作者：
Bolton, VN
中科院分区：
文献类型：
--
作者：
Almeida, PA;Bolton, VN
研究了温度波动对人卵母细胞纺锤体完整性和染色体组织的影响，以及这种影响对所产生的单性生殖的染色体构成的影响。用α-微管蛋白抗体对340个卵母细胞进行免疫细胞化学染色，其中502个卵母细胞被孤雌激活。卵母细胞在室温下暴露2、10、30min后，纺锤体断裂的发生率分别为77%(n=26)、72%(n=18)和89%(n=19)，染色体分散率分别为50%、56%和52.6%。当卵母细胞在室温下暴露2分钟后回到37℃时，这些效应被逆转，但在10分钟或30分钟后不能。降温对卵母细胞的孤雌激活率(2min：67%，n=27；10min：68%，n=28；30min：54%，n=35)和孤雌激活卵裂率(30min：53%，n=19)均有影响。温度引起的卵母细胞纺锤体损伤(70%，50/63)与暴露在室温下的卵母细胞发育的单倍体染色体异常(56%，23/41；p<；0.01)有直接联系。
The effect of temperature fluctuation on spindle integrity and chromosomal organisation in the human oocyte, and the consequences of such effects on the chromosomal constitution of resulting parthenotes, were investigated. A total of 340 oocytes were stained immunocytochemically with an antibody to a-tubulin, and 502 were activated parthenogenetically. Exposure of oocytes to room temperature for 2, 10 or 30 min caused disruption of the spindle in 77% (n = 26), 72% (n = 18) and 89% (n = 19) of cases respectively, with evidence of chromosomal dispersal in 50%, 56% and 52.6% respectively. These effects were reversed when oocytes were returned to 37 degrees C after exposure to room temperature for 2 min, but not after 10 min or 30 min. Temperature reduction affected rates of parthenogenetic activation of oocytes (2 min: 67%, n = 27; 10 min: 68%, n = 28; 30 min: 54%, n = 35) and cleavage of resulting parthenotes, but only if oocytes were exposed to room temperature for 30 min (30 min: 53%, n = 19). There is a direct association between temperature-induced spindle damage in the oocyte (70%, 50 of 63) and chromosomal abnormalities in parthenotes developed from oocytes exposed to room temperature (56%, 23 of 41; p < 0.01).