A translocated human c-myc oncogene is altered in a conserved coding sequence.
A translocated human c-myc oncogene is altered in a conserved coding sequence.
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易位的人类 c-myc 癌基因的保守编码序列发生改变。
DOI：
10.1073/pnas.83.9.2939
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发表时间：
1986
影响因子：
11.1
通讯作者：
P. Leder
中科院分区：
文献类型：
--
作者：
W. Murphy;J. Sarid;R. Taub;T. Vasicek;J. Battey;G. Lenoir;P. Leder
我们已经克隆并表征了一个c-myc（现命名为MYC）癌基因，该基因已被易位到伯基特淋巴瘤细胞系中免疫球蛋白重链基因座的mu开关区。易位的断点发生在c-myc基因的第一个内含子内，从而将未易位的第一个外显子与两个编码外显子分开。来自易位基因的转录由第一内含子内的隐蔽启动子产生，其产生438个核苷酸的非翻译5'区。由c-myc基因编码的蛋白质的氨基酸序列已被显著改变。特别是，一组移码突变的补偿改变了在人类、小鼠和鸡c-myc基因以及人类N-myc和L-myc癌基因中紧密保守的蛋白质区域中的一串24个氨基酸。尽管如此，突变的基因在大鼠胚胎成纤维细胞病灶形成试验中保留了降低的转化能力。
We have cloned and characterized a c-myc (now designated MYC) oncogene that had been translocated into the mu switch region of the immunoglobulin heavy chain locus in a Burkitt lymphoma cell line. The breakpoint of the translocation occurs within the first intron of the c-myc gene, thereby separating the untranslocated first exon from the two coding exons. Transcription from the translocated gene arises from a cryptic promoter within the first intron, which produces a 438-nucleotide untranslated 5' region. The amino acid sequence of the protein encoded by the c-myc gene has been substantially altered. In particular, a compensating set of frameshift mutations alters a string of 24 amino acids in a region of the protein tightly conserved in human, mouse, and chicken c-myc genes as well as in the human N-myc and L-myc oncogenes. Despite this, the mutated gene retains a reduced transforming ability in a rat embryo fibroblast focus-formation assay.