Coordinate responses of transcription factors to ecdysone during programmed cell death in the anterior silk gland of the silkworm, Bombyx mori
Coordinate responses of transcription factors to ecdysone during programmed cell death in the anterior silk gland of the silkworm, Bombyx mori
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DOI：
10.1111/j.1365-2583.2006.00641.x
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发表时间：
2006-06-01
影响因子：
2.6
通讯作者：
Sakurai, S.
中科院分区：
文献类型：
--
作者：
Sekimoto, T.;Iwami, M.;Sakurai, S.
家蚕前丝腺 (ASG) 中的程序性细胞死亡 (PCD) 由 20-羟基蜕皮激素 (20E) 触发。我们检查了第五龄期 20E 对 11 个转录因子基因的表达谱和影响，以确定它们是否表现出果蝇 PCD 中的分层控制。结果表明，EcR-A 和 usp-2，而不是 EcR-B1 或 usp-1，可能是蜕皮激素受体复合物的成分。 E75A、BHR3 和三种 BR-C 亚型（但 E75B 除外）的上调似乎与 PCD 的诱导相关。 beta FTZ-F1 在 PCD 执行期间不表达。因此，家蚕ASG中的基因控制与果蝇唾液腺中的基因控制不同，尽管两种组织在蛹变态时都经历了响应20E的PCD。
Programmed cell death (PCD) in Bombyx mori anterior silk glands (ASGs) is triggered by 20-hydroxyecdysone (20E). We examined the expression profiles and effects of 20E on 11 transcription factor genes in the fifth instar to determine whether they demonstrate the hierarchical control seen in Drosophila PCD. Results indicate that EcR-A and usp-2, but not EcR-B1 or usp-1, may be components of the ecdysone receptor complex. Up-regulation of E75A, BHR3, and three BR-C isoforms, but not E75B, appeared to be associated with the induction of PCD. beta FTZ-F1 was not expressed during PCD execution. Thus, gene control in B. mori ASGs differs from that in Drosophila salivary glands, despite both tissues undergoing PCD in response to 20E at pupal metamorphosis.