The C. elegans HGF/plasminogen-like protein SVH-1 has protease-dependent and -independent functions.
The C. elegans HGF/plasminogen-like protein SVH-1 has protease-dependent and -independent functions.
复制标题
线虫 HGF/纤溶酶原样蛋白 SVH-1 具有蛋白酶依赖性和非蛋白酶依赖性功能。
DOI：
10.1016/j.celrep.2014.10.056
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发表时间：
2014
期刊：
影响因子：
8.8
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Hisamoto;N.;Li;C.;Yoshida;M.;Matsumoto;K.
肝细胞生长因子（HGF）和纤溶丝氨酸蛋白酶纤溶酶原可能是从脊椎动物的共同祖先进化而来的。这很难确定，因为在其他血统中没有祖先形式被确定。在秀丽隐杆线虫中，HGF/纤溶酶原样蛋白SVH-1通过HGF受体同源物SVH-2调节轴突再生。在这项研究中，我们发现svh-1和svh-2基因在许多无脊椎动物中是保守的。我们还表明，SVH-1有一个额外的功能，独立的SVH-2，控制幼虫的生长。SVH-1蛋白酶活性是幼虫生长所必需的，但不是轴突再生。svh-1的缺失会导致FBL-1蛋白（一种细胞外基质（ECM）组分纤蛋白）在咽部周围的异常积累，而这种生长缺陷会被FBL-1缺失部分抑制。这些结果表明，SVH-1作为一个生长因子和蛋白酶，他们也提供了深入了解动物的HGF/纤溶酶原的演变。
Hepatocyte growth factor (HGF) and fibrinolytic serine protease plasminogen may have evolved from a common ancestor in vertebrates. This has been hard to ascertain, as no ancestral form has been identified in other lineages. InCaenorhabditis elegans,an HGF/plasminogen-like protein SVH-1 regulates axon regeneration via the HGF receptor homolog SVH-2. In this study, we report that both thesvh-1andsvh-2genes are conserved in many invertebrates. We also show that SVH-1 has an additional function, independent of SVH-2, which controls larval growth. SVH-1 protease activity is essential for larval growth, but not for axon regeneration. Deletion ofsvh-1causes abnormal accumulation of FBL-1 protein, an extracellular matrix (ECM) component fibulin, around the pharynx, and this growth defect is partially suppressed by FBL-1 depletion. These results suggest that SVH-1 acts as both a growth factor and a protease, and they also provide insights into the evolution of HGF/plasminogen in animals.