Effect of tension-force on plasminogen activator activity from human periodontal ligament cells.
Effect of tension-force on plasminogen activator activity from human periodontal ligament cells.
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DOI：
10.1111/j.1600-0765.1997.tb00539.x
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发表时间：
1997-04
影响因子：
3.5
通讯作者：
M. Yamaguchi;N. Shimizu;Y. Ozawa;K. Saito;S. Miura;H. Takiguchi;T. Iwasawa;Y. Abiko
中科院分区：
文献类型：
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作者：
M. Yamaguchi;N. Shimizu;Y. Ozawa;K. Saito;S. Miura;H. Takiguchi;T. Iwasawa;Y. Abiko
纤溶酶原激活剂(PA)-纤溶酶蛋白水解系统由于其参与多种生物活性和涉及组织破坏的病理状态，最近受到了相当大的关注。在严重的牙周炎中，过度的机械应力如咬合创伤与牙槽骨丢失有关。因此，机械应力可能涉及咬合损伤通过激活pa -纤溶蛋白水解系统降解细胞外基质。我们检测了机械应力对人PDL细胞中PA活性、组织型(t) PA、尿激酶型(u) PA和PA抑制剂-1 （PAI-1）基因表达的影响。人PDL细胞在柔性底培养板上培养，并置于Flexercell Strain Unit上。细胞在9%和18%伸长率下弯曲6个周期（5 s应变，5 s松弛），持续5天。施加拉力诱导应激PDL细胞的PA活性显著高于非应激对照，并且呈时间和幅度依赖性（p < 0.001，方差分析）。Western-blot分析显示，高水平的活性是由于tPA而不是uPA。通过RT-PCR检测，应激PDL细胞中tPA mRNA的基因表达在第5天增加。这些发现提示tPA可能参与牙周代谢对机械应力的反应。
The plasminogen activator (PA)-plasmin proteolytic system has recently received considerable attention because of its participation in a wide variety of biological activities and in pathological conditions involving tissue destruction. Excessive mechanical stress such as occlusal trauma is associated with alveolar bone loss in severe periodontitis. Therefore, mechanical stress may involve degradation of the extracellular matrix by occlusal trauma through activation of the PA-plasmin proteolytic system. We examined the effects of mechanical stress on PA activity, gene expressions of tissue type (t) PA, urokinase type (u) PA and PA inhibitor-1 (PAI-1) in human PDL cells. Human PDL cells were cultured on flexible-bottomed culture plates and placed on a Flexercell Strain Unit. The cells were flexed at 6 cycles (5 s strain, 5 s relaxation) at 9% and 18% elongation for 5 d. Application of tension-force induced significantly higher PA activity in stressed PDL cells than in non-stressed controls, and did so in a time- and magnitude-dependent manner (p < 0.001, ANOVA). Western-blot analysis revealed that the high level of activity was due to tPA and not uPA. Gene expression of tPA mRNA in stressed PDL cells, as examined by RT-PCR, increased on d 5. These findings suggest that tPA may be involved in periodontal metabolism in response to mechanical stress.