DEVELOPMENT OF TRYPANOSOMA-CRUZI IN MACROPHAGES INVITRO - INTERACTION WITH LYSOSOMES AND HOST-CELL FATE
DEVELOPMENT OF TRYPANOSOMA-CRUZI IN MACROPHAGES INVITRO - INTERACTION WITH LYSOSOMES AND HOST-CELL FATE
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DOI：
10.1017/s0031182000000597
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发表时间：
1980-01-01
期刊：
影响因子：
2.4
通讯作者：
KLOETZEL, J
中科院分区：
文献类型：
--
作者：
MILDER, R;KLOETZEL, J
在光学显微镜和电镜水平上研究了小鼠腹膜巨噬细胞与Y型克氏t细胞血流形态的相互作用。在细胞单层被寄生虫感染之前或之后，用Thorotrast标记溶酶体的方法显示，在锥虫内化后不久，次级溶酶体与吞噬体融合。尽管如此，24小时后，大多数寄生虫不再在液泡中，而是在宿主细胞质中自由地躺着，活跃地繁殖。在这个时候，直到寄生虫逃逸到外部环境的96小时前不久，大多数被寄生的细胞都没有受到致命的伤害，这是台普兰蓝染料排除试验所显示的。只有当寄生虫被释放到外部培养基中时，这种情况才会逆转，被感染的巨噬细胞才会吸收染料。
The interaction between mouse peritoneal macrophages and Y strain T. cruzi bloodstream forms was studied at optical microscopical and EM levels. The method of marking lysosomes with Thorotrast, either before or after infection of cell monolayers with parasites, revealed that secondary lysosomes fused with phagosomes shortly after trypanosome interiorization. Despite this, 24 h later most parasites were no longer in a vacuole but lay free within the host cell cytoplasm, multiplying actively. At this time, and up to shortly before 96 h when parasites escaped to the external milieu, most parasitized cells were not lethally injured, as revealed by the Trypan blue dye exclusion test. Only when parasites were released into the external medium was this situation reversed, and infected macrophages took up the dye.