Phenotype MicroArrays for high-throughput phenotypic testing and assay of gene function
Phenotype MicroArrays for high-throughput phenotypic testing and assay of gene function
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DOI：
10.1101/gr.186501
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发表时间：
2001-07-01
期刊：
影响因子：
7
通讯作者：
Panomitros, E
中科院分区：
文献类型：
--
作者：
Bochner, BR;Gadzinski, P;Panomitros, E
大肠杆菌被用作模型细胞系统来测试和验证一种名为表型微阵列（PMs）的新技术。PM技术是一种同时检测大量细胞表型的高通量技术。它包括预先配置的孔阵列，其中每个孔测试不同的细胞表型，以及一个自动化仪器，该仪器连续监测和记录阵列中所有孔中细胞的反应。例如，只需将细胞悬浮液移液到7个微孔板阵列中，就可以检测近700种大肠杆菌的表型。pmms可用于直接测定基因变化对细胞的影响，特别是基因敲除。在这里，我们提供了六种菌株的表型分析数据，并表明我们可以检测到预期的表型，以及在某些情况下，意外的表型。
The bacterium Escherichia coli is used as a model cellular system to test and validate a new technology called Phenotype MicroArrays (PMs). PM technology is a high-throughput technology for simultaneous testing of a large number of cellular phenotypes. It consists of preconfigured well arrays in which each well tests a different cellular phenotype and an automated instrument that continuously monitors and records the response of the cells in all wells of the arrays. For example, nearly 700 phenotypes of E. coli can be assayed by merely pipetting a cell suspension into seven microplate arrays. PMs can be used to directly assay the effects of genetic changes on cells, especially gene knock-outs. Here, we provide data on phenotypic analysis of six strains and show that we can detect expected phenotypes as well as, in some cases, unexpected phenotypes.