Phospholipase Cε regulates ovulation in Caenorhabditis elegans
Phospholipase Cε regulates ovulation in Caenorhabditis elegans
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DOI：
10.1016/j.ydbio.2004.06.024
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发表时间：
2004-10-01
影响因子：
2.7
通讯作者：
Kataoka, T
中科院分区：
文献类型：
--
作者：
Kariya, K;Bui, YK;Kataoka, T
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Phospholipase Cepsilon (PLCepsilon) is a novel class of phosphoinositide-specific PLC with unknown physiological functions. Here, we present the first genetic analysis of PLCepsilon in an intact organism, the nematode Caenorhabditis elegans. Ovulation in C. elegans is dependent on an inositol 1,4,5-trisphosphate (IP3) signaling pathway activated by the receptor tyrosine kinase LET-23. We generated deletion mutants of the gene, plc-1, encoding C. elegans PM. We observed a novel ovulation phenotype whereby oocytes are trapped in the spermatheca due to delayed dilation of the spermatheca-uterine valve. The expression of plc-1 in the adult spermatheca is consistent with its involvement in regulation of ovulation. On the other hand, we failed to observe genetic interaction of plc-1 with let-23-mediated IP3 signaling pathway genes, suggesting a complex mechanism for control of ovulation. (C) 2004 Elsevier Inc. All rights reserved.