FGF SIGNALING AND TARGET RECOGNITION IN THE DEVELOPING XENOPUS VISUAL-SYSTEM
FGF SIGNALING AND TARGET RECOGNITION IN THE DEVELOPING XENOPUS VISUAL-SYSTEM
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DOI：
10.1016/0896-6273(95)90091-8
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发表时间：
1995-11-01
期刊：
影响因子：
16.2
通讯作者：
HOLT, CE
中科院分区：
文献类型：
--
作者：
MCFARLANE, S;MCNEILL, L;HOLT, CE
我们报道了爪蟾视网膜神经节细胞的生长锥表达成纤维细胞生长因子受体（FGFRs），并且bFGF刺激培养的视网膜神经元的神经突延伸。此外，bFGF在发育中的视神经束中含量丰富，但在视神经顶盖中含量较少。为了测试FGF信号在体内是否在轴突引导中发挥作用，我们将bFGF外源性应用于“暴露脑”制剂中发育中的视神经通路。fgf治疗的视网膜轴突在视神经束中正常导航，但大多数轴突在尖端边缘异常转向并绕过目标。我们的研究结果暗示FGF信号在目标识别中起作用，并表明顶盖中bFGF水平的降低导致到达轴突的生长减慢。
We report that the growth cones of Xenopus retinal ganglion cells express fibroblast growth factor receptors (FGFRs) and that bFGF stimulates neurite extension from cultured retinal neurons. Furthermore, bFGF is abundant in the developing optic tract but is reduced in the optic tectum. To test whether FGF signaling plays a role in axonal guidance in vivo, bFGF was exogenously applied to the developing optic pathway in ''exposed brain'' preparations. FGF-treated retinal axons navigate normally through the optic tract, but the majority veer aberrantly at the tectal border and bypass the target. Our results implicate FGF signaling in target recognition and suggest that diminished levels of bFGF in the tectum cause arriving axons to slow their growth.