CELL-WALL RECEPTOR FOR BACTERIOPHAGE MU-G(+)
CELL-WALL RECEPTOR FOR BACTERIOPHAGE MU-G(+)
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DOI：
10.1128/jb.160.1.299-303.1984
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发表时间：
1984-01-01
影响因子：
3.2
通讯作者：
KAMP, D
中科院分区：
文献类型：
--
作者：
SANDULACHE, R;PREHM, P;KAMP, D
噬菌体Mu DNA中的可逆G片段控制噬菌体的宿主范围。根据G片段的方向，产生两种类型的噬菌体颗粒，G（+）和G（-），其识别不同的细胞表面受体。Mu G（+）受体定位于革兰氏阴性菌的脂多糖（LPS）上。对不同LPS核心类型和对Mu G（+）具有抗性的突变体的分析表明，大肠杆菌K-12上的主要受体位点位于GlcNac β 1的cnt dot。. cntdot. . cntdot. 6 GC α- 2Glc α-部分位于LPS的外端。Mu与E. coliK-12与无关单链DNA噬菌体St-1。与Mu相关的噬菌体D108和与Mu无关但含有同源可逆DNA片段的噬菌体P1和P7具有不同的受体需求。由于它们也以不同的构型与末端葡萄糖结合，因此它们吸附并感染E。coli K-12菌株，具有不完整的LPS核心。
The invertible G segment in phage Mu DNA controls the host range of the phage. Depending on the orientation of the G segment, 2 types of phage particles, G(+) and G(-), are produced which recognize different cell surface receptors. The receptor for Mu G(+) was located in the lipopolysaccharide (LPS) of gram-negative bacteria. The analysis of different LPS core types and of mutants that were made resistant to Mu G(+) shows that the primary receptor site on Escherichia coli K-12 lies in the GlcNac.beta.1 .cntdot. .cntdot. .cntdot. 6 Gc.alpha.-2 Glc.alpha.- part at the outer end of the LPS. Mu shares this receptor site in E. coli K-12 with the unrelated single-stranded DNA phage St-1. Phage D108, which is related to Mu, and phages P1 and P7, which are unrelated to Mu but contain a homologous invertible DNA segment, have different receptor requirements. Since they also bind to terminal glucose in a different configuration, they adsorb to and infect E. coli K-12 strains with an incomplete LPS core.