Orphan neuropeptide NocII, a putative pronociceptin maturation product, stimulates locomotion in mice
Orphan neuropeptide NocII, a putative pronociceptin maturation product, stimulates locomotion in mice
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DOI：
10.1097/00001756-199702100-00025
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发表时间：
1997-02-10
期刊：
影响因子：
1.7
通讯作者：
Costentin, J
中科院分区：
文献类型：
--
作者：
Florin, S;Suaudeau, C;Costentin, J
NocII是一种十七肽，其序列位于伤害感受肽（nociceptin）的前体多肽原伤害感受肽（pronociceptin）中伤害感受肽（新发现的ORL1受体的天然激动剂）的紧下游。由于NocII的序列被推定的转化酶切除位点框定，并且在鼠和人类物种中完全保守，我们试图确定这种孤儿神经肽是否具有生理学意义，即在体内具有中心生物活性。脑室注射10和100 ng的NocII可增加小鼠的运动能力。然而，与刺激运动的水平和垂直（直立）分量的伤害感受素不同，NocII只影响水平分量。NocII的运动兴奋作用似乎在很大程度上取决于多巴胺传递，因为它完全被D1或D2多巴胺受体拮抗剂SCH 23390和氟哌啶醇逆转。NocII不改变孔须测试中探索的孔的数量，这表明，与痛敏肽不同，孤儿肽不影响小鼠的探索行为。
NocII is a heptadecapeptide whose sequence lies immediately downstream of nociceptin, the newly discovered natural agonist of the ORL1 receptor, in pronociceptin, nociceptin's precursor polypeptide. Since the sequence of NocII is framed by putative convertase excision sites and it totally conserved across murine and human species, we have sought to determine whether this orphan neuropeptide might by physiologically significant, i.e. endowed with central biological activity in vivo. Intracerebroventricular administration of 10 and 100 ng of NocII increased locomotion in mice. However, unlike nociceptin, which stimulates both the horizontal and vertical (rearing) components of locomotion, NocII affected only the horizontal component. The motor stimulant action of NocII appears to depend largely on dopamine transmission since it is totally reversed by the D1 or the D2 dopamine receptor antagonists SCH 23390 and haloperidol. NocII does not modify the number of explored holes in the hole beard test, indicating that, unlike nociceptin, the orphan peptide does not affect exploratory behavior in mice.