Scavenger receptor B protects shrimp from bacteria by enhancing phagocytosis and regulating expression of antimicrobial peptides
Scavenger receptor B protects shrimp from bacteria by enhancing phagocytosis and regulating expression of antimicrobial peptides
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清道夫受体 B 通过增强吞噬作用和调节抗菌肽的表达来保护虾免受细菌侵害。
DOI：
10.1016/j.dci.2015.02.001
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发表时间：
2015-07-01
影响因子：
2.9
通讯作者：
Wang, Jin-Xing
中科院分区：
文献类型：
--
作者：
Bi, Wen-Jie;Li, Dian-Xiang;Wang, Jin-Xing
清道夫受体 (SR) 通过识别病原体相关分子模式 (PAMP) 参与先天免疫，并通过与损伤相关分子模式 (DAMP) 相互作用参与疾病的发病机制。 SR 在无脊椎动物先天免疫中的作用仍有待阐明。在这里，我们从库鲁玛虾（Marsupenaeus japonicus）中鉴定出 B 类清道夫受体，命名为 MjSR-B1。重组MjSR-B1以钙依赖性方式凝集细菌并结合脂多糖和脂磷壁酸。敲低MjSR-B1后，日本国参对鳗弧菌和金黄色葡萄球菌的细菌清除能力和吞噬能力均受损，并且多个吞噬相关基因下调。抗菌肽的表达水平也下调。 MjSR-B1 的过度表达导致细菌清除率、吞噬率增强以及吞噬相关和抗菌肽基因的上调。然而，突变体 MjSR-B1 Delta C 的过度表达（缺乏 MjSR-B1 的羧基尾部）没有这些效果。我们的结果表明，MjSR-B1 可以通过促进吞噬作用和增强抗菌肽的表达来保护虾免受细菌侵害。 (C) 2015 Elsevier Ltd. 保留所有权利。
Scavenger receptors (SRs) are involved in innate immunity through recognizing pathogen-associated molecular patterns (PAMPs) and in pathogenesis of diseases through interactions with damage-associated molecular patterns (DAMPs). The roles of SRs in invertebrate innate immunity still need to be elucidated. Here we identify a class B scavenger receptor from kuruma shrimp, Marsupenaeus japonicus, designated MjSR-B1. The recombinant MjSR-B1 agglutinated bacteria in a calcium dependent manner and bound lipopolysaccharide and lipoteichoic acid. After knockdown of MjSR-B1, both the bacterial clearance and phagocytotic ability of M. japonicus against V. anguillarum and S. aureus were impaired, and several phagocytosis related genes were downregulated. The expression levels of antimicrobial peptides were also downregulated. Overexpression of MjSR-B1 led to enhanced bacterial clearance, phagocytosis rate and upregulation of phagocytosis-related and antimicrobial peptide genes. However, overexpression of mutant MjSR-B1 Delta C, which lacks the carboxyl tail of MjSR-B1, had none of these effects. Our results indicate that MjSR-B1 can protect shrimp from bacteria by promoting phagocytosis and by enhancing the expression of antimicrobial peptides. (C) 2015 Elsevier Ltd. All rights reserved.