ALKALINE INORGANIC PYROPHOSPHATASE ACTIVITY OF MAMMALIAN-CELL ALKALINE PHOSPHATASE
ALKALINE INORGANIC PYROPHOSPHATASE ACTIVITY OF MAMMALIAN-CELL ALKALINE PHOSPHATASE
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DOI：
10.1042/bj1050155
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发表时间：
1967-01-01
影响因子：
4.1
通讯作者：
GRIFFIN, MJ
中科院分区：
文献类型：
--
作者：
COX, RP;GILBERT, P;GRIFFIN, MJ
从哺乳动物细胞培养物中制备的碱性磷酸酶具有碱性无机焦磷酸酶活性。碱性磷酸酶和碱性无机焦磷酸酶的同时纯化表明，这两种活性似乎都与一种蛋白质有关；随着抗体浓度的增加，滴定一定量的酶制剂，按比例沉淀碱性磷酸酶和无机焦磷酸酶的活性；免疫电泳结果表明，沉淀蛋白带具有碱性磷酸酶活性，也具有焦磷酸酶活性；半胱氨酸对碱性磷酸酶活性的抑制作用组织化学方法证实这两种酶的亚细胞定位相似；在哺乳动物细胞培养中，激素和底物诱导碱性磷酸酶活性，使无机焦磷酸酶活性几乎平行上升。
Alkaline phosphatase " prepared from mammalian cell cultures had alkaline inorganic pyrophosphatase activity. Both of these activities appear to be associated with a single protein, as demonstrated by concomitant purification of alkaline phosphatase and alkaline inorganic pyrophosphatase; proportional precipitation of alkaline phosphatase and inorganic pyrophosphatase activities by titrating constant amounts of an enzyme preparation with increasing concentration of antibody; immune electrophoresis, which showed that precipitin bands that have alkaline phosphatase activity also have pyrophosphatase activity; inhibition of pyrophosphatase activity by cysteine, an inhibitor of alkaline phosphatase activity; similar subcellular localization of the 2 enzyme activities as demonstrated by histochemical methods; hormonal and substrate induction of alkaline phosphatase activity in mammalian cell cultures, which produced a nearly parallel rise in inorganic pyrophosphatase activity.