Anti-hyperglycemic activity of an aqueous extract from flower buds of Cleistocalyx operculatus (Roxb.) Merr and Perry
Anti-hyperglycemic activity of an aqueous extract from flower buds of Cleistocalyx operculatus (Roxb.) Merr and Perry
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DOI：
10.1271/bbb.60373
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发表时间：
2007-01-01
影响因子：
1.6
通讯作者：
Van Chuyen, Nguyen
中科院分区：
文献类型：
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作者：
Mai, Truong Tuyet;Van Chuyen, Nguyen
对越南5种饮料用植物进行了筛选，发现了一种闭锁花（Cleistocalx operculatus）。山梨花蕾提取物对α -葡萄糖苷酶的抑制活性最高。因此，我们在体内和体外研究了越南一种常用的饮料制备材料——锁骨苞（CO）花蕾水提取物的抗高血糖作用。在体外，CO提取物抑制大鼠肠道麦芽糖酶和蔗糖酶活性，IC50值分别为0.70和0.47 mg/ml。这些值低于番石榴叶提取物（GE； IC50分别为0.97和1.28 mg/ml）。麦芽糖对正常小鼠和stz诱导的糖尿病大鼠餐后血糖的测定。结果表明，CO （500mg/kg bw）对血糖的降低作用略低于阿卡波糖（25mg /kg bw），但比GE （500mg/kg bw）更有效。在为期8周的实验中，与未处理的糖尿病大鼠相比，500 mg CO/kg bw/day处理的STZ糖尿病大鼠血糖水平明显降低。因此，一氧化碳被认为是预防和治疗糖尿病的一种有前途的材料。
A screening of 5 plants used for making drinks in Vietnam revealed a Cleistocalx operculatus (Roxb.) Merr and Perry flower bud extract to have the highest inhibitory activity against the alpha-glucosidase enzyme. The anti-hyperglycemic effects of an aqueous extract from flower buds of Cleistocalyx operculatus (CO), a commonly used material for drink preparation in Vietnam, were therefore investigated in vitro and in vivo. In vitro, the CO extract inhibited the rat-intestinal maltase and sucrase activities, with IC50 values of 0.70 and 0.47 mg/ml, respectively. These values are lower than those for a guava leaf extract (GE; IC50 0.97 and 1.28 mg/ml, respectively). Postprandial blood glucose testing of normal mice and STZ-induced diabetic rats by maltose. loading (2 g/kg body weight (bw)) showed that the blood glucose reduction with CO (500mg/kg bw) was slightly less than that with acarbose (25 mg/kg bw) but was more potent than that with GE (500 mg/kg bw). In an 8-week experiment, the blood glucose level of STZ diabetic rats treated with 500 mg of CO/kg bw/day was markedly decreased in comparison with that of non-treated diabetic rats. Consequently, CO is considered to be a promising material for preventing and treating diabetes.