CELL VOLUMES OF NORMAL AND MALIGNANT MONONUCLEAR-CELLS
CELL VOLUMES OF NORMAL AND MALIGNANT MONONUCLEAR-CELLS
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DOI：
10.1136/jcp.34.10.1083
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发表时间：
1981-01-01
影响因子：
3.4
通讯作者：
DAVEY, FR
中科院分区：
文献类型：
--
作者：
CHAPMAN, EH;KUREC, AS;DAVEY, FR
本文测定了20例正常人外周血中单个核细胞、T淋巴细胞、B淋巴细胞和单核细胞的细胞体积，并与14例慢性淋巴细胞白血病、20例急性淋巴细胞白血病和18例非霍奇金S淋巴瘤的肿瘤淋巴样细胞进行了比较。将单个核细胞与绵羊红细胞进行玫瑰花环反应，然后用Ficoll和泛影葡盐组成的梯度离心法获得正常T细胞。通过将单核细胞粘贴到塑料培养皿中制备单核细胞群，通过从单核细胞群中去除T淋巴细胞和单核细胞而获得B细胞。细胞体积在H4型Channelyzer库尔特计数器上测定。正常人T淋巴细胞平均细胞体积(MCV)小于B淋巴细胞，B淋巴细胞平均MCV小于单核细胞平均MCV(P<；0.05)。CLL患者淋巴细胞的平均MCV小于正常B细胞的平均MCV(P<0.01)。ALL患者淋巴细胞的平均MCV大于正常外周血淋巴细胞的平均MCV(P<；0.01)。淋巴母细胞的大小在ALL病例内部和之间表现出很大的差异。大多数NHL患者淋巴细胞的MCV大于反应性淋巴结和正常人外周血淋巴细胞的MCV。观察到肿瘤细胞的MCV与RapPaport的分类之间存在关联。淋巴样细胞体积的测定可能有助于各种淋巴增生性疾病患者的诊断和预后。
The cell volumes of mononuclear cells, T lymphocytes, B lymphocytes, and monocytes from the peripheral blood of 20 normal individuals were compared to neoplastic lymphoid cells from 14 patients with chronic lymphocytic leukemia (CLL), 20 individuals with acute lymphocytic leukemia (ALL), and 18 cases of non-Hodgkin''s lymphoma (NHL). Normal T cells were obtained by rosetting mononuclear cells with sheep erythrocytes followed by centrifugation on a gradient composed of Ficoll and diatrizoate salts. Monocyte populations were prepared by adhering mononuclear cells to plastic dishes and B cells were obtained by the depletion of T lymphocytes and monocytes from a mononuclear cell population. Cell volumes were determined on a Coulter Counter Model H4 Channelyzer. In normals, the average mean cell volume (MCV) of T lymphocytes was smaller than B lymphocytes and the average MCV of B lymphocytes was smaller than the average MCV of monocytes (P < 0.05). The average MCV of lymphocytes from patients with CLL was smaller than the average MCV of normal B cells (P < 0.01). The average MCV of lymphoblasts from cases of ALL was larger than the average MCV of normal peripheral blood lymphocytes (P < 0.01). The size of lymphoblasts showed great variation within and among cases of ALL. The MCV of lymphocytes from most cases of NHL was larger than the MCV of lymphocytes from reactive lymph nodes and from the peripheral blood of normal individuals. An association was observed between the MCV of neoplastic cells and the classification according to Rappaport. The measurement of lymphoid cell volumes may be helpful in the diagnosis and prognosis of patients with a variety of lymphoproliferative disorders.