Improving the diagnostic efficacy of squamous cell carcinoma antigen for oral squamous cell carcinoma via saponin disruption of serum extracellular vesicles
Improving the diagnostic efficacy of squamous cell carcinoma antigen for oral squamous cell carcinoma via saponin disruption of serum extracellular vesicles
复制标题
通过皂苷破坏血清细胞外囊泡提高鳞状细胞癌抗原对口腔鳞状细胞癌的诊断效能
DOI：
10.1016/j.cca.2021.12.012
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发表时间：
2022
影响因子：
5
通讯作者：
Chen Fuxiang
中科院分区：
文献类型：
--
作者：
Yang Meng;Ding Jieying;Luo Qingqiong;Chen Xu;Chen Fuxiang
背景鳞状细胞癌抗原（SCCA）对口腔鳞状细胞癌（OSCC）的诊断价值不足。最近，细胞外囊泡（EV）已显示出提高诊断效率的巨大潜力。然而，限制EV应用的主要挑战之一是缺乏临床上合适的分离方法用于囊泡内蛋白detection.MethodsSaponin膜被用来破坏血清EV释放囊泡内SCCA到血清中，绕过EV的纯化过程。结果皂苷破坏EVS细胞膜后，血清SCCA浓度明显升高，与对照组比较差异有统计学意义（P < 0. 05）。血清SCCA诊断OSCC的曲线下面积（AUC）为0.6444（95%CI，0.5595至0.7293）。血清EVs来源的SCCA的诊断AUC为0.7969（95%CI，0.735 ~ 0.8588）。结论经皂苷破坏的血清EVs可提高SCCA对口腔鳞癌的诊断效率，为EVs囊泡内蛋白的检测提供了一种简便、快速、高通量的方法，具有很好的临床应用前景。
BackgroundThe diagnostic value of squamous cell carcinoma antigen (SCCA) for oral squamous cell carcinoma (OSCC) is insufficient. Recently, extracellular vesicles (EVs) have displayed great potential for improving diagnostic efficacy. However, one of the main challenges that restricts the application of EVs is the lack of a clinically suitable separation method for the intra-vesicular protein detection.MethodsSaponin was used to destroy serum EVs membranes for releasing the intra-vesicular SCCA into the serum, circumventing the purification process of EVs. The concentrations of SCCA were measured and compared in 113 healthy people and 73 OSCC patients pre- and post-saponin treatment.ResultsThe concentration of serum SCCA significantly increased after saponin destroyed the membrane of EVs. The area under the curve (AUC) of serum SCCA for OSCC diagnosis was 0.6444 (95% CI, 0.5595 to 0.7293). The diagnostic AUC of serum EVs-derived SCCA reached 0.7969 (95% CI, 0.735 to 0.8588).ConclusionsThe results suggested that serum EVs disrupted by saponin could improve the diagnostic efficacy of SCCA for OSCC, which provides a simple, rapid, and high-throughput method to detect the intra-vesicular proteins of EVs and holds great potential for clinical application.