Myocardin expression during avian embryonic heart development requires the endoderm but no independent of BMP signaling
Myocardin expression during avian embryonic heart development requires the endoderm but no independent of BMP signaling
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DOI：
10.1002/dvdy.21393
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发表时间：
2008-01-01
影响因子：
2.5
通讯作者：
Antin, Parker B.
中科院分区：
文献类型：
--
作者：
Warkman, Andrew S.;Yatskievych, Tatiana A.;Antin, Parker B.
心肌素是一种血清反应因子辅助因子，在调节心脏和平滑肌发育中起重要作用。为了研究心肌蛋白在心脏发育早期阶段的功能，我们分离了心肌蛋白的鸡直系同源物，并表征了其在汉堡和汉密尔顿阶段3和15之间的表达。在第4阶段，myocardin的成绩单中检测到的侧面和胚外中胚层，逐渐成为本地化的心前中胚层和分化的心肌，也被认为是在平滑肌细胞的发展血管丛。令人惊讶的是，发育中的鸡胚胎内的心肌蛋白表达先于同源结构域转录因子Nkx2.5的表达。胚胎解剖研究表明，来自内胚层的信号是心前中胚层内心肌素表达所必需的。然而，与Nkx2.5不同的是，myocardin的表达不受骨形态发生蛋白（BMP）信号转导的调节。这些结果表明，心肌素在心前中胚层的初始表达是由一个信号通路，是平行的，独立的，Nkx2.5的表达。
Myocardin, a serum response factor cofactor, plays an important role in regulating heart and smooth muscle development. To investigate myocardin function during early stages of heart development, we isolated the chicken orthologue of myocardin and characterized its expression between Hamburger and Hamilton stages 3 and 15. At stage 4, myocardin transcripts are detected in the lateral and extraembryonic mesoderm, become progressively localized to the precardiac mesoderm and the differentiated myocardium and are also seen in smooth muscle cells of the developing vascular plexus. Surprisingly, myocardin expression within the developing chicken embryo precedes that of the homeodomain transcription factor Nkx2.5. Embryonic dissection studies demonstrate that signals from the endoderm are required for myocardin expression within the precardiac mesoderm. However, unlike Nkx2.5, myocardin expression is not regulated by bone morphogenetic protein (BMP) signaling. These results suggest that initial expression of myocardin in the precardiac mesoderm is regulated by a signalling pathway that is parallel to, and independent of, Nkx2.5 expression.