Photoactivated miR-148b-nanoparticle conjugates improve closure of critical size mouse calvarial defects.
Photoactivated miR-148b-nanoparticle conjugates improve closure of critical size mouse calvarial defects.
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DOI：
10.1016/j.actbio.2014.10.010
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发表时间：
2015-01
影响因子：
9.7
通讯作者：
Hayes, Daniel J.
中科院分区：
文献类型：
--
作者：
Qureshi, Ammar T.;Doyle, Andrew;Chen, Cong;Coulon, Diana;Dasa, Vinod;Del Piero, Fabio;Levi, Benjamin;Monroe, W. Todd;Gimble, Jeffrey M.;Hayes, Daniel J.
提供分化的时间控制的诱导系统具有通过精确调节伤口愈合和组织修复过程来改善外科重建和再生医学的结果的潜力。本研究的目的是证明通过光暴露激活的纳米配制的microRNA（miRNA）缀合物可以导致体内人脂肪源性基质/干细胞（hASC）的诱导成骨分化。偶联物PC-miR-148 b-SNP，一种通过光不稳定接头与银纳米颗粒（SNP）连接的miRNA-148 b模拟物，用于调节基因表达，以改善雄性CD-1裸纯合小鼠右顶骨上钻取的临界尺寸缺损的闭合。将PC-miR-148 b-SNP缀合物添加到hASC中并加载到基质胶或聚己内酯（PCL）支架中导致不同水平的缺损愈合。4周和12周后，3-D显微计算机断层扫描重建图像显示PCL支架中非光活化和光活化缀合物的统计学显著缺陷闭合分别为3.83 ± 1.19%至5.46 ± 2.01%和6.54 ± 4.28%至32.53 ± 8.3%。在光活化缀合物的横切面中用H&E和Masson三色染色证实了结果。胶原纤维染色在12周时最大，此时其达到与天然颅骨大致相同的密度和厚度。这项技术提供了一个平台，可以与其他miRNAs一起使用，这些miRNAs积极地控制着负责再生和伤口愈合过程的途径。
Inducible systems providing temporal control of differentiation have the potential to improve outcomes in surgical reconstruction and regenerative medicine by precise modulation of wound healing and tissue repair processes. The aim of this study was to demonstrate that nanoformulated microRNA (miRNA) conjugates activated via photo exposure can lead to the induced osteogenic differentiation of human adipose-derived stromal/stem cells (hASCs) in vivo. The conjugate PC-miR-148b–SNP, a mimic of miRNA-148b tethered to silver nanoparticles (SNPs) via a photolabile linker, was used to modulate gene expression for improved closure of a critical size defect drilled on the right parietal bone of male CD-1 nude homozygous mice. The PC-miR-148b–SNP conjugates added to hASCs and loaded to either Matrigel or polycaprolactone (PCL) scaffolds resulted in different levels of healing of the defect. After 4 and 12 weeks, 3-D micro-computed tomography reconstructed images indicate statistically significant defect closure from 3.83 ± 1.19% to 5.46 ± 2.01% and 6.54 ± 4.28% to 32.53 ± 8.3% for non-photoactivated and photoactivated conjugates, respectively, in the PCL scaffolds. The results were confirmed with H&E and Masson’s Trichrome stains in the transverse sections of photoactivated conjugates. Collagen fiber staining was greatest at 12 weeks when it reached approximately the same density and thickness as the native calvarium. This technology provides a platform that can be used with other miRNAs that actively govern the pathways responsible for regenerative and wound healing processes.
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影响因子：
3.1
作者：
Forman, Julianne;Dietrich, Marilyn;Monroe, W. Todd
通讯作者：
Monroe, W. Todd
使用表达抗 miR-31 的骨髓基质干细胞和聚（癸二酸甘油酯）支架修复临界尺寸的骨缺损。
影响因子：
3.1
作者：
Deng, Yuan;Bi, Xiaoping;Fan, Xianqun
通讯作者：
Fan, Xianqun
影响因子：
--
作者：
Mendes, SC;Tibbe, JM;Van Blitterswijk, CA
通讯作者：
Van Blitterswijk, CA
影响因子：
4
作者：
Kapinas, Kristina;Kessler, Catherine B.;Delany, Anne M.
通讯作者：
Delany, Anne M.
miR-31 修饰的脂肪组织来源的干细胞在修复大鼠临界大小的颅骨缺损中的作用。
影响因子：
14
作者：
Deng, Yuan;Zhou, Huifang;Fan, Xianqun
通讯作者：
Fan, Xianqun