Longispora urticae sp nov., isolated from rhizosphere soil of Urtica urens L., and emended descriptions of the species Longispora albida and Longispora fulva
Longispora urticae sp nov., isolated from rhizosphere soil of Urtica urens L., and emended descriptions of the species Longispora albida and Longispora fulva
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Longispora urticae sp nov.，从 Urtica urens L. 的根际土壤中分离出来，并修正了 Longispora albida 和 Longispora fulva 种的描述
DOI：
10.1099/ijsem.0.002288
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发表时间：
2017
影响因子：
2.8
通讯作者：
Xiang Wensheng
中科院分区：
文献类型：
--
作者：
Piao Chenyu;Jin Liying;Zhao Junwei;Liu Chongxi;Zhao Yue;Wang Xiangjing;Xiang Wensheng
从荨麻根际土壤中分离到2株革兰氏阳性好氧放线菌，命名为NEAU-PCY-3 T和NEAU-PCY-4。采自中国东北辽宁省鞍山市。16 S rRNA基因序列分析表明，两个菌株的16 S rRNA基因序列相似性为99.9%，与Longispora fulvaDSM 45356 T（98.7，98.9%）和Longispora albidaJCM 11711 T（97.1，97.2%）的相似性最高。 基于16 S rRNA基因序列的系统发育分析表明，这两株菌与长孢霉属（Longispora）同属一个谱系，均含有MK-10（H4）和MK-10（H6）两种甲基萘醌类化合物。发现细胞壁肽聚糖含有内消旋-二氨基庚二酸、d-谷氨酸、甘氨酸和l-丙氨酸。全细胞酶解产物主要含有半乳糖、核糖和木糖。磷脂谱包含二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇、几种糖脂和几种未知脂质。菌株NEAU-PCY-3 T的主要脂肪酸为iso-C16：0、iso-C17：0、anteiso-C17：0和C18：1ω5c。 菌株NEAU-PCY-3 T和NEAU-PCY-4之间的DNA-DNA杂交值为83.6± 0.4%，与其亲缘关系最近的长孢霉属（Longispora）菌株之间的杂交值远低于70%，支持它们代表一个不同的基因组种。 一系列的表型特征也将菌株与其密切相关的物种区分开来，这是仅有的两个有效分离的长孢子种。根据遗传学、化学分类学和表型特征，菌株NEAU-PCY-3 T和NEAU-PCY-4被分类为长孢属一新种的代表，该新种命名为Longispora urticaesp.十一是建议。模式菌株为NEAU-PCY-3 T（=DSM 105119 T =CCTCC AA 2017017 T）。
Two Gram-stain-positive, aerobic actinomycete strains, designated NEAU-PCY-3Tand NEAU-PCY-4, were isolated from rhizosphere soil ofUrtica urensL. collected from Anshan, Liaoning Province, northeast PR China. The 16S rRNA gene sequence analysis showed that the two strains exhibited 99.9 % 16S rRNA gene sequence similarity with each other and that they were most closely toLongispora fulvaDSM 45356T(98.7, 98.9 %) andLongispora albidaJCM 11711T(97.1, 97.2 %). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the two strains were located in the same lineage and formed a cluster with the genusLongispora .Both strains were observed to contain MK-10(H4) and MK-10(H6) as the predominant menaquinones. The cell wall peptidoglycan was found to containmeso-diaminopimelic acid,d-glutamic acid, glycine andl-alanine. Whole-cell hydrolysates mainly contained galactose, ribose and xylose. The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, several glycolipids and several unknown lipids. The major cellular fatty acids for strain NEAU-PCY-3Twere iso-C16 : 0, iso-C17 : 0, anteiso-C17 : 0and C18 : 1ω5c. The DNA–DNA hybridization value between strains NEAU-PCY-3Tand NEAU-PCY-4 was 83.6±0.4 %, and the values between the two strains and their closest phylogenetic relatives, belonging to the genusLongispora, were well below 70 %, supporting that they represented a distinct genomic species. An array of phenotypic characteristics also differentiated the strains from their closely related species, the only two validly publishedLongisporaspecies. On the basis of the genetic, chemotaxonomic and phenotypic properties, strains NEAU-PCY-3Tand NEAU-PCY-4 were classified as representatives of a novel species of the genusLongispora, for which the nameLongispora urticaesp. nov. is proposed. The type strain is NEAU-PCY-3T(=DSM 105119T=CCTCC AA 2017017T).