"Musical dish" efficiently induces osteogenic differentiation of mesenchymal stem cells through music derived microstretch with variable frequency.
"Musical dish" efficiently induces osteogenic differentiation of mesenchymal stem cells through music derived microstretch with variable frequency.
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DOI：
10.1002/btm2.10291
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发表时间：
2022-05
影响因子：
7.4
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非均匀微拉伸（NUMS）自然发生在体内真实的骨组织中，但其深远的影响尚未得到证实。为了探索NUMS和静态拉伸（均匀拉伸[US]）对细胞的生物学效应，开发了一种新的“音乐碟”装置。音乐信号用于向细胞提供NUMS。与US和未处理的对照组相比，NUMS组的细胞中形成了更多沿着细胞长轴排列的应力纤维，但细胞形态没有任何改变。全转录组测序显示NUMS处理后细胞的成骨分化增强。NUMS组中的细胞显示骨相关基因的表达更高，而与干性和其他谱系相关的基因下调。我们的研究结果提供了深入了解NUMS和US对干细胞成骨分化的生物学效应，表明微机械刺激对成骨的有益影响。新开发的装置为NUMS衍生康复技术的发展提供了基础，以促进骨愈合。
Nonuniform microstretching (NUMS) naturally occurs in real bone tissues in vivo, but its profound effects have not been identified yet. In order to explore the biological effects of NUMS and static stretch (uniform stretch [US]) on cells, a new “musical dish” device was developed. Musical signal was used to provide NUMS to cells. More stress fibers, arranging along the long axis of cells, were formed throughout the cells under NUMS, compared with US and untreated control group, although cell morphology did not show any alteration. Whole transcriptome sequencing revealed enhanced osteogenic differentiation of cells after NUMS treatment. Cells in the NUMS group showed a higher expression of bone‐related genes, while genes related to stemness and other lineages were down‐regulated. Our results give insights into the biological effects of NUMS and US on stem cell osteogenic differentiation, suggesting beneficial effects of micromechanical stimulus for osteogenesis. The newly developed device provides a basis for the development of NUMS derived rehabilitation technology to promote bone healing.