Plasma versus serum levels of osteopontin and mesothelin in patients with malignant mesothelioma-Which is best?
Plasma versus serum levels of osteopontin and mesothelin in patients with malignant mesothelioma-Which is best?
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DOI：
10.1016/j.lungcan.2011.02.007
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发表时间：
2011-10-01
期刊：
影响因子：
5.3
通讯作者：
Robinson, Bruce W. S.
中科院分区：
文献类型：
--
作者：
Creaney, Jenette;Yeoman, Deborah;Robinson, Bruce W. S.
背景资料：基于血液的恶性间皮瘤（MM）的标志物，特别是可溶性间皮素和骨桥蛋白，目前具有很大的临床意义。由于存在对血清中骨桥蛋白与血浆中骨桥蛋白的敏感性的一些关注，我们将它们在相同患者群体中与间皮素进行比较。可溶性间皮素和骨桥蛋白浓度通过商业测定法在来自66名胸膜MM患者，47名非恶性石棉相关肺或胸膜疾病患者，结果：MM患者血清和血浆中可溶性间皮素和骨桥蛋白水平均显著高于肺良性疾病和胸膜良性疾病患者。在相对于良性疾病患者的95%特异性水平下，在呈现症状时血清和血浆中的间皮素的灵敏度为67%，血浆中的骨桥蛋白的灵敏度为40%，MM患者的血清中的灵敏度为20%。结合血清间皮素和血浆骨桥蛋白标志物使用logistic回归模型并没有显着增加的面积下receiver operator curve.Conclusion：血浆骨桥蛋白具有上级诊断准确性血清。由于血液样本类型的选择对可溶性间皮素敏感性的影响有限，因此应在疑似患有间皮瘤的患者中收集血浆用于生物标志物评价。皇冠版权所有（C）2011由爱思唯尔爱尔兰有限公司出版。保留所有权利。
Background: Blood-based markers for malignant mesothelioma (MM), particularly soluble mesothelin and osteopontin, are currently of great clinical interest. As there is some concern about the sensitivity of osteopontin in serum versus plasma, we compared them in the same patient population to mesothelin.Methods: Soluble mesothelin and osteopontin concentrations were determined by commercial assays in blood samples from 66 patients with pleural MM, 47 patients with non-malignant asbestos-related lung or pleural disease, 42 patients with other benign pleural and lung diseases and 21 patients with lung cancer.Results: Soluble mesothelin and osteopontin in serum and plasma were significantly elevated in MM patients compared to patients with benign lung and pleural disease. At a level of specificity of 95% relative to patients with benign disease, the sensitivity of mesothelin in serum and plasma at presentation with symptoms was 67%, and for osteopontin in the plasma was 40% and in the serum was 20% for MM patients. Combining the serum mesothelin and plasma osteopontin markers using a logistic regression model did not significantly increase the area under the receiver operator curve.Conclusion: Plasma osteopontin has a superior diagnostic accuracy to serum. As the choice of blood sample type has limit effect on soluble mesothelin sensitivity, plasma should be collected for biomarker evaluation in patients suspected of having mesothelioma. Crown Copyright (C) 2011 Published by Elsevier Ireland Ltd. All rights reserved.