Prevalence and quantification of the uncommon Archaea phylotype Thermoplasmata in chronic periodontitis
Prevalence and quantification of the uncommon Archaea phylotype Thermoplasmata in chronic periodontitis
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DOI：
10.1016/j.archoralbio.2014.05.011
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发表时间：
2014-08-01
影响因子：
3
通讯作者：
Shu, Rong
中科院分区：
文献类型：
--
作者：
Li, Chao Lun;Jiang, Yun Tao;Shu, Rong
目的：慢性牙周炎是牙周组织的一种慢性炎症性疾病，是由某些类型的细菌和古菌侵入引起的，以口腔甲烷杆菌为优势古菌。在这项研究中，我们调查了新发现的古菌热原菌在慢性牙周炎患者中的患病率和数量。方法：对49例慢性牙周炎患者和45例牙周健康者进行龈下菌斑检测。对菌斑样品DNA提取液进行16S rRNA基因扩增，对古菌和热原菌进行定性分析，并对所有样品进行实时聚合酶链反应（PCR）定量分析。结果：慢性牙周炎患者中古生菌的常规PCR检出率为69.4%，而实时PCR检出率为87.8%。在对照组中，有三个样本被检测为阳性，但在定性分析中均未得到证实。巢式PCR结果显示，慢性牙周炎组热原体感染率为18.4%，定量PCR结果为24.5%。热原菌的流行率明显低于总古菌的流行率。古菌和热原菌的相对丰度因样品而异。热原菌不是龈下牙菌斑中占优势的古菌。牙周炎患者的临床参数中，探牙深度与古生菌检出率呈正相关。结论：古生菌的存在与慢性牙周炎的发生密切相关。热原菌是牙周感染中的一种小型古细菌。(C) 2014 Elsevier Ltd.版权所有。
Objective: Chronic periodontitis is a chronic inflammatory disease of the periodontal tissues and is caused by invasion of certain types of bacteria and Archaea, with Methanobrevibacter oralis as the predominant archaeon. In this study, we investigated the prevalence and quantity of the newly discovered Archaea phylotype Thermoplasmata in patients with chronic periodontitis.Methods: Subgingival plaque samples were obtained from 49 patients with chronic periodontitis and 45 periodontally healthy subjects. Qualitative analyses of Archaea and class Thermoplasmata were carried out by amplification of 16S rRNA genes in DNA extracts from plaque samples, and all the samples were quantitatively analyzed by real-time polymerase chain reaction (PCR).Results: The prevalence of Archaea in patients with chronic periodontitis was 69.4% according to the conventional PCR results, but was 87.8% according to real-time PCR. In the control group, three samples were detected as positive, but none of these were confirmed in qualitative analyses. The prevalence of class Thermoplasmata was 18.4% by nested PCR and 24.5% by quantitative PCR in the chronic periodontitis group. The prevalence of Thermoplasmata was significantly lower than that of total Archaea. The relative abundances of Archaea and Thermoplasmata varied among samples. Thermoplasmata were not the predominant archaeons in the subgingival dental plaque. Among the clinical parameters of patients with periodontitis, probing depth was positively associated with Archaea detection.Conclusions: The existence of Archaea was correlated closely with the presence of chronic periodontitis. Thermoplasmata represented a minor archaeon in periodontal infection. (C) 2014 Elsevier Ltd. All rights reserved.