Age-related changes on the morphology of the otoconia
Age-related changes on the morphology of the otoconia
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DOI：
10.1097/01.mlg.0000217238.84401.03
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发表时间：
2006-06-01
期刊：
影响因子：
2.6
通讯作者：
Kim, Lee Suk
中科院分区：
文献类型：
--
作者：
Jang, Yoon Seok;Hwang, Chan Ho;Kim, Lee Suk
目的/假设：虽然使用场发射扫描电镜（FESEM）研究耳锥体形态的报道很多，但关于耳锥体形态随年龄变化的报道很少。本研究的目的是利用FESEM观察大鼠耳锥体形态随年龄的变化。研究设计：使用实验动物进行实验室研究。方法：采用FESEM技术，研究青年（1周龄）、中年（6月龄）和老年（23月龄）三组大鼠耳锥体形态学的年龄相关性变化。结果：幼龄大鼠和老年大鼠脑室耳郭的大小差异较大，但我们发现各组的囊状耳郭在大小上没有明显的区域分离。在年龄最大的大鼠中，两个黄斑中的许多耳蜗的身体都有凹坑、裂缝、穿透，最终破碎成几块碎片。尽管本组耳膜体有退行性变，但耳膜端切面光滑，切面相交线清晰。老年大鼠脑室黄斑外缘多见巨大耳郭。我们直接观察到老年组连接丝和耳锥碎片变弱或断裂。结论：老年大鼠耳蜗及连接丝与耳蜗碎片变性最明显。本研究的年龄相关的耳聋形态学变化可能有助于我们了解起源的特发性良性阵发性位置性眩晕。
Objectives/Hypothesis: Although there are numerous reports on otoconial morphology using field-emission scanning electron microscopy (FESEM), there are few reports regarding the changes of otoconial morphology with aging. The aim of the current study was to investigate changes in otoconial morphology in rats according to age, using FESEM. Study Design: Laboratory study using experimental animals. Methods: We investigated age-related changes in otoconial morphology using FESEM in three groups of rats: young (1 wk old), middle-aged (6 mo old), and aged (23 mo old). Results: There was great size variation in utricular otoconia in the young and aged rats, but we found no clear regional separation of saccular otoconia in all groups based on size. In the oldest rats, the bodies of many otoconia in both maculae were pitted, fissured, penetrated, and eventually broken into several fragments. However, the terminal facets were smooth and the lines of intersection of facets were sharp, despite the degenerated bodies of the otoconia in this group. Giant otoconia were discovered frequently on the outer margin of the utricular maculae in aged rats. We directly observed weakened or broken linking filaments and otoconial fragments in the aged group. Conclusion: The oldest rats showed the most degeneration of otoconia and linking filaments with otoconial fragments. This study of age-related morphologic changes in otoconia might help us understand the origin of idiopathic benign paroxysmal positional vertigo.