Brain natriuretic peptide is produced in cardiac fibroblasts and induces matrix metalloproteinases
Brain natriuretic peptide is produced in cardiac fibroblasts and induces matrix metalloproteinases
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DOI：
10.1161/01.res.0000046234.73401.70
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发表时间：
2002-12-13
影响因子：
20.1
通讯作者：
Burnett, JC
中科院分区：
文献类型：
--
作者：
Tsuruda, T;Boerrigter, G;Burnett, JC
心脏成纤维细胞(CFs)产生细胞外基质蛋白，参与心脏重塑。目前尚不清楚脑利钠肽(BNP)是否由我们合成，以及BNP是否参与细胞外基质代谢的调节。在本研究中，我们研究了BNP在成年犬体内的产生，以及BNP及其信号系统在胶原合成和基质金属蛋白酶(MMPs)激活中的作用。放射免疫分析显示BNP1-32以每48小时11.2+/-1.0pg/10(5)细胞的速度分泌到培养液中(平均+/-扫描电子显微镜)。脑钠素分泌量显著高于对照组(P
Cardiac fibroblasts (CFs) produce extracellular matrix proteins and participate in the remodeling of the heart. It is unknown if brain natriuretic peptide (BNP) is synthesized by Us and if BNP participates in the regulation of extracellular matrix turnover. In this study, we examined the production of BNP in adult canine Us and the role of BNP and its signaling system on collagen synthesis and on the activation of matrix metalloproteinases (MMPs). BNP mRNA was detected in CFs, and a specific radioimmunoassay demonstrated that BNP1-32 was secreted into the media at a rate of 11.2 +/- 1.0 pg/10(5) cells per 48 hours (mean +/- SEM). The amount of BNP secretion was significantly (P