ProSA-web: interactive web service for the recognition of errors in three-dimensional structures of proteins.
ProSA-web: interactive web service for the recognition of errors in three-dimensional structures of proteins.
复制标题
DOI：
10.1093/nar/gkm290
复制
发表时间：
2007-07
影响因子：
14.9
通讯作者：
Sippl, Manfred J
中科院分区：
文献类型：
--
作者：
Wiederstein, Markus;Sippl, Manfred J
结构生物学的一个主要问题是识别蛋白质结构实验和理论模型中的错误。 Prosa程序（蛋白质结构分析）是一种已建立的工具，具有较大的用户群，并且经常用于实验蛋白质结构以及结构预测和建模的改进和验证。蛋白质结构的分析通常是一种困难而繁琐的运动。这里提供的新服务是经典Prosa程序的直接且易于使用的扩展，该计划利用了基于交互式Web的应用程序的优势来显示分数和能量图，这些分数和能量图突出了蛋白质结构中发现的潜在问题。特别地，在所有已知蛋白质结构的背景下显示蛋白质的质量评分，并且在3D分子观看器中显示并突出显示结构的有问题部分。该服务专门解决了从X射线分析，NMR光谱和理论计算获得的蛋白质结构验证中遇到的需求。 prosa-web可以在https://prosa.services.came.sbg.ac.at上访问
A major problem in structural biology is the recognition of errors in experimental and theoretical models of protein structures. The ProSA program (Protein Structure Analysis) is an established tool which has a large user base and is frequently employed in the refinement and validation of experimental protein structures and in structure prediction and modeling. The analysis of protein structures is generally a difficult and cumbersome exercise. The new service presented here is a straightforward and easy to use extension of the classic ProSA program which exploits the advantages of interactive web-based applications for the display of scores and energy plots that highlight potential problems spotted in protein structures. In particular, the quality scores of a protein are displayed in the context of all known protein structures and problematic parts of a structure are shown and highlighted in a 3D molecule viewer. The service specifically addresses the needs encountered in the validation of protein structures obtained from X-ray analysis, NMR spectroscopy and theoretical calculations. ProSA-web is accessible at https://prosa.services.came.sbg.ac.at