Dendrigraft poly-L-lysines delivery of DNA vaccine effectively enhances the immunogenic responses against H9N2 avian influenza virus infection in chickens
Dendrigraft poly-L-lysines delivery of DNA vaccine effectively enhances the immunogenic responses against H9N2 avian influenza virus infection in chickens
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树突状聚赖氨酸递送 DNA 疫苗有效增强鸡对 H9N2 禽流感病毒感染的免疫原性反应
DOI：
10.1016/j.nano.2020.102209
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Li Zejun
中科院分区：
文献类型：
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作者：
Zhao Kai;Rong Guangyu;Teng Qiaoyang;Li Xuesong;Lan Hailing;Yu Lu;Yu Shuang;Jin Zheng;Chen Guangping;Li Zejun
可生物降解的纳米材料可以保护抗原不被降解，促进细胞吸收，增强免疫反应。将优化的H9 N2亚型禽流感病毒血凝素（HA）基因片段插入pCAGGS载体，构建了真核表达质粒pCAGS-opti 441-HA。将pCAGGS-opti 441-HA与树枝状多聚赖氨酸（DGL）连接，构建了pCAGGS-opti 441-HA/DGL。DGL不仅能保护pCAGS-opti 441-HA不被降解，而且转染效率高。用pCAGS-opti 441-HA/DGL免疫鸡可诱导强烈的细胞免疫应答。免疫后第3周，免疫鸡的IFN-γ、IL-2水平和淋巴细胞转化率均升高。免疫鸡T淋巴细胞活化增殖，CD 3 + CD 4+、CD 4 +/CD 8+比值升高，对H9 N2亚型禽流感病毒攻击具有保护作用。本研究为新型禽流感疫苗的研制提供了方法，为安全高效的基因载体的研制提供了理论依据。
Biodegradable nanomaterials can protect antigens from degradation, promote cellular absorption, and enhance immune responses. We constructed a eukaryotic plasmid [pCAGGS-opti441-hemagglutinin (HA)] by inserting the optimized HA gene fragment of H9N2 AIV into the pCAGGS vector. The pCAGGS-opti441-HA/DGL was developed through packaging the pCAGGS-opti441-HA with dendrigraft poly-l-lysines (DGLs). DGL not only protected the pCAGGS-opti441-HA from degradation, but also exhibited high transfection efficiency. Strong cellular immune responses were induced in chickens immunized with the pCAGGS-opti441-HA/DGL. The levels of IFN-γ and IL-2, and lymphocyte transformation rate of the vaccinated chickens increased at the third week post the immunization. For the vaccinated chickens, T lymphocytes were activated and proliferated, the numbers of CD3+CD4+ and CD4+/CD8+ increased, and the chickens were protected completely against H9N2 AIV challenge. This study provides a method for the development of novel AIV vaccines, and a theoretical basis for the development of safe and efficient gene delivery carriers.