The Expression of Manganese Superoxide Dismutase Gene from Nelumbo nucifera Responds Strongly to Chilling and Oxidative Stresses
The Expression of Manganese Superoxide Dismutase Gene from Nelumbo nucifera Responds Strongly to Chilling and Oxidative Stresses
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DOI：
10.1111/j.1744-7909.2008.00790.x
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发表时间：
2009-03-01
影响因子：
11.4
通讯作者：
Huang, Shangzhi
中科院分区：
文献类型：
--
作者：
Li, Wen;Qi, Lin;Huang, Shangzhi
从圣莲（Nelumbo nucifera Gaertn.）胚轴中克隆到一个锰超氧化物歧化酶（Mn-SOD）基因，命名为NnMSD 1。NnMSD 1蛋白包含Mn-SOD蛋白家族的所有保守残基，包括四个共有金属结合结构域和一个线粒体靶向信号肽。Southern杂交分析表明，在圣莲中存在两个Mn-SOD基因。NnMSD 1在种子发育过程中在发育中的胚轴中高度表达，但仅在发育的早期阶段出现在子叶中，并且在胚胎发生的晚期在子叶中变得不可检测。还研究了NnMSD 1基因在萌发胚轴中响应于各种应激如热激、低温和暴露于应激相关化学物质的表达。热激强烈抑制NnMSD 1基因的表达，而NnMSD 1的转录水平在低温胁迫处理强烈增加。H2 O2在萌发的胚轴中也高度诱导表达的增加。结果表明，NnMSD 1基因的表达模式不同发育轴和子叶，NnMSD 1基因的表达强烈响应低温和氧化胁迫。
A manganese superoxide dismutase (Mn-SOD) gene, NnMSD1, was identified from embryonic axes of the sacred lotus (Nelumbo nucifera Gaertn.). The NnMSD1 protein contains all conserved residues of the Mn-SOD protein family, including four consensus metal binding domains and a signal peptide for mitochondrial targeting. Southern blot analysis suggests the existence of two Mn-SOD genes in sacred lotus. NnMSD1 was highly expressed in developing embryonic axes during seed development, but appeared in cotyledons only at the early stage of development and became undetectable in the cotyledons during late embryogenesis. The expression of the NnMSD1 gene in germinating embryonic axes, in response to various stresses such as heat shock, chilling, and exposure to stress-related chemicals, was also studied. Heat shock strongly inhibited the expression of the NnMSD1 gene, whereas the NnMSD1 transcript level increased strongly in chilling stress treatment. An increase in expression was also highly induced by H2O2 in germinating embryonic axes. The results suggest that the expression pattern of the NnMSD1 gene differed between developing axes and cotyledons, and that the NnMSD1 gene expression responds strongly to chilling and oxidative stress.