Structures of an active type III-A CRISPR effector complex.
Structures of an active type III-A CRISPR effector complex.
复制标题
活性Ⅲ-A型CRISPR效应复合体的结构。
DOI：
10.1016/j.str.2022.05.013
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发表时间：
2022-08-04
期刊：
影响因子：
5.7
通讯作者：
Mondragon, Alfonso
中科院分区：
文献类型：
--
作者：
Smith, Eric M.;Ferrell, Se;Tokars, Valerie L.;Mondragon, Alfonso
簇状规则间隔短回文重复序列(CRISPR)及其相关蛋白(CA)为许多原核生物提供了抵抗遗传物质入侵的适应性免疫系统。III型CRISPR系统的独特之处在于它们可以同时降解RNA和DNA。作为对入侵核酸的反应，它们会产生环状寡腺苷，充当第二信使，激活细胞核酸酶，帮助免疫反应。在这里，我们提出了七个单颗粒冷冻-EM结构的III-A型表皮葡萄球菌CRISPR效应复合体。这些结构揭示了完整的表皮葡萄球菌效应器复合体在apo、ATP结合、同源靶RNA结合和非同源靶RNA结合状态下的作用，并说明了效应器复合体如何结合和呈现crRNA。与靶RNA结合的复合体在RNA后切割状态下捕获III-A型效应器复合体。ATP结合结构给出了ATP如何与Cas10结合以促进环状寡腺苷生产的细节。史密斯等人在apo、ATP结合以及自身和非自身靶RNA结合状态下，存在完整的和催化活性的表皮葡萄球菌III-A型CRISPR效应复合体的冷冻-EM结构。这些结构提供了关于复杂的整体结构和化学计量、靶RNA识别和Cas10生产的环状寡腺苷的信息。
Clustered regularly interspaced short palindromic repeats (CRISPR) and their CRISPR associated proteins (Cas) provide many prokaryotes with an adaptive immune system against invading genetic material. Type III CRISPR systems are unique in that they can degrade both RNA and DNA. In response to invading nucleic acids they produce cyclic oligoadenylates that act as secondary messengers, activating cellular nucleases that aid in the immune response. Here we present seven single particle cryo-EM structures of the Type III-A Staphylococcus epidermidis CRISPR effector complex. The structures reveal the intact S. epidermidis effector complex in an apo, ATP bound, cognate target RNA bound, and non-cognate target RNA bound states and illustrate how the effector complex binds and presents crRNA. The complexes bound to target RNA capture the Type III-A effector complex in a post RNA cleavage state. The ATP bound structures give details about how ATP binds to Cas10 to facilitate cyclic oligoadenylate production. Smith et al. present cryo-EM structures of intact and catalytically active S. epidermidis Type III-A CRISPR effector complexes in apo, ATP bound, and self and non-self target RNA bound states. The structures provide information on the complex overall architecture and stoichiometry, target RNA recognition, and cyclic oligoadenylate production by Cas10.
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影响因子：
16
作者：
Jia, Ning;Mo, Charlie Y.;Patel, Dinshaw J.
通讯作者：
Patel, Dinshaw J.
影响因子：
14.9
作者：
Dorsey, Bryan W.;Huang, Lei;Mondragon, Alfonso
通讯作者：
Mondragon, Alfonso
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
DOI：
10.1107/s2059798318006551
发表时间：
2018-06-01
期刊：
Acta crystallographica. Section D, Structural biology
影响因子：
--
作者：
Afonine PV;Poon BK;Read RJ;Sobolev OV;Terwilliger TC;Urzhumtsev A;Adams PD
通讯作者：
Adams PD
影响因子：
0.8
作者：
Chou-Zheng, Lucy;Hatoum-Aslan, Asma
通讯作者：
Hatoum-Aslan, Asma