Antifungal resistance of Candida glabrata vaginal isolates and development of a quantitative reverse transcription-PCR-based azole susceptibility assay
Antifungal resistance of Candida glabrata vaginal isolates and development of a quantitative reverse transcription-PCR-based azole susceptibility assay
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DOI：
10.1128/aac.00462-08
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发表时间：
2008-09-01
影响因子：
4.9
通讯作者：
Trama, Jason P.
中科院分区：
文献类型：
--
作者：
Gygax, Scott E.;Vermitsky, John-Paul;Trama, Jason P.
建立了一种多重定量逆转录- pcr方法检测光秃念珠菌（Candida glabrata）对唑的耐药性。抗性定义为CDR1表达增加>= 3倍，与金标准肉汤微量稀释法相比，该方法被证明具有100%的敏感性和95%的特异性。
A multiplex quantitative reverse transcription-PCR assay was developed to detect azole resistance in Candida glabrata, an important opportunistic pathogen that develops resistance rapidly. Resistance was defined as a >= 3-fold increase in CDR1 expression by this assay, which proved to be 100% sensitive and 95% specific in comparison to the gold standard broth microdilution assay.