Transforming growth factor-beta1 protein, proliferation and apoptosis of oval cells in acetylaminofluorene-induced rat liver regeneration.
Transforming growth factor-beta1 protein, proliferation and apoptosis of oval cells in acetylaminofluorene-induced rat liver regeneration.
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DOI：
10.3346/jkms.1999.14.5.531
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发表时间：
1999-10
影响因子：
4.5
通讯作者：
Suh KS
中科院分区：
文献类型：
--
作者：
Park DY;Suh KS
在三分之二的肝部分切除术(PHX)前给予2-乙酰氨基荧烯(2-AAF)可抑制肝细胞的增殖并刺激卵圆细胞的增殖。本研究的目的是通过联合2-AAF和PHX引起的选择性肝损伤来检测卵圆细胞的行为和相关的转化生长因子-β1(TGF-β1)蛋白的表达。我们还研究了转化生长因子-β1的表达与卵圆细胞增殖和凋亡的时间关系。卵圆细胞从汇管区出现，并随着时间的推移向汇管区周围和中带肝实质内散开，数量增多。平滑肌肌动蛋白(SMA)和转化生长因子-β1免疫组化染色显示，转化生长因子-β1阳性细胞为SMA阳性的肝星状细胞(HSCs)。随着卵圆细胞的增殖，SMA阳性的HSCs产生的转化生长因子-β1表达增加，随后卵圆细胞的凋亡率达到高峰。提示HSCs产生的转化生长因子-β1与卵圆细胞的增殖和凋亡密切相关，并在2-AAF诱导的肝再生中阻止卵圆细胞活化和肝实质重塑中发挥作用。
Administering of 2-acetylaminofluorene (2-AAF) before a two-thirds partial hepatectomy (PHx) results in suppression of hepatocyte proliferation and stimulation of oval cell proliferation. The objectives of this study was to examine the oval cell behaviour and associated transforming growth factor-beta1 (TGF-beta1) protein expression by combining 2-AAF with selective hepatic damage caused by PHx. We also studied the temporal relationship between TGF-beta1 expression, and proliferation and apoptosis of oval cells. Oval cells emerged from the portal areas and became more numerous with time fanning out into the periportal and midzonal hepatic parenchyma. Both smooth muscle actin (SMA) and TGF-beta1 immunostain revealed that TGF-beta1-positive cells were SMA-positive hepatic stellate cells (HSCs). Coinciding with the proliferation of oval cells, an increase expression of TGF-beta1 produced by SMA-positive HSCs was observed, thereafter apoptosis of oval cells reached its peak. This result implicated that TGF-beta1 produced by HSCs is intimately associated with proliferation and apoptosis of oval cells, and plays a role in the cessation of oval cell activation and remodeling of liver parenchyma in 2-AAF induced liver regeneration.
DOI：
10.1111/j.1349-7006.1990.tb02552.x
发表时间：
1990-03
期刊：
Japanese journal of cancer research : Gann
影响因子：
--
作者：
Ito N;Kawata S;Tamura S;Takaishi K;Saitoh R;Tarui S
通讯作者：
Tarui S