A comparative analysis of antibacterial activity, dynamics, and effects of silver ions and silver nanoparticles against four bacterial strains
A comparative analysis of antibacterial activity, dynamics, and effects of silver ions and silver nanoparticles against four bacterial strains
复制标题
银离子和纳米银对四种细菌的抗菌活性、动力学和效果的比较分析
DOI：
10.1016/j.ibiod.2017.07.015
复制
发表时间：
2017-09-01
影响因子：
4.8
通讯作者：
Huang, Xiao-Mo
中科院分区：
文献类型：
--
作者：
Li, Wen-Ru;Sun, Ting-Li;Huang, Xiao-Mo
虽然银离子和银纳米粒子(AgNPs)都具有很好的抗菌活性，但人们认为AgNPs的抗菌活性要强于银离子。在这项研究中，我们比较分析了银离子和两种类型的AgNPs对四种细菌的活性、动态和效果。银离子、AgNPs(I)和AgNPs(II)对大肠杆菌的最低抑菌浓度(MIC)分别为0.5、1和2 mU g/mL，对铜绿假单胞菌的最低抑菌浓度(MIC)分别为1、2和8 mU g/mL，对金黄色葡萄球菌的最低抑菌浓度(MIC)分别为1、2和4 mU g/mL，对表皮葡萄球菌的最低抑菌浓度(MIC)分别为1、2和2 mU g/mL。本实验结果表明，Ag+具有比AgNPs(I)和AgNPs(II)更强的抗菌活性。抗菌动态曲线显示，银离子、AgNPs(I)和AgNPs(II)均以浓度依赖的方式延长了4种细菌的生长滞后期。此外，透射电子显微镜观察表明，经2mU g/mL银离子和AgNPs处理的细菌细胞在5h内大部分被破坏，所有银离子、AgNPs(I)和AgNPs(II)都能引起细菌细胞的严重损伤。细菌的鞭毛受损甚至消失，会导致运动障碍。细胞表面可见许多孔洞或缝隙，导致细胞质和大分子渗漏，最终导致细胞死亡。我们的结果表明，银离子具有类似的作用模式，但抗菌活性略高于银纳米粒子对细菌细胞的作用。(C)2017爱思唯尔有限公司。保留所有权利。
Although both silver ions and silver nanoparticles (AgNPs) have perfect antibacterial activity, but it was assumed that AgNPs have stronger activity than that of silver ions. In this study, we make a comparative analysis of activity, dynamics, and effects of silver ions and two types of AgNPs against four bacterial strains. The minimum inhibitory concentrations (MICs) of silver ions, AgNPs (I) and AgNPs (II) were 0.5, 1 and 2 mu g/mL against E. coli, 1, 2 and 8 mu g/mL against P. aeruginosa, 1, 2 and 4 mu g/mL against S. aureus, and 1, 2 and 2 mu g/mL against S. epidermidis respectively. This experimental results showed that Ag+ have stronger antibacterial activity than that of AgNPs (I) and AgNPs(II). Antibacterial dynamic curves revealed all the silver ions, AgNPs (I), and AgNPs (II) prolonged the growth lag phase of all four bacteria in a concentration-dependent manner. Furthermore, transmission electronic microscopy (TEM) observation showed that a major part of bacterial cells treated with 2 mu g/mL of silver ion and AgNPs were destroyed within 5 h. The transmission electron microscopy (TEM) observation indicated that all the silver ions, AgNPs (I), and AgNPs (II) can induce severe damage in bacterial cells. The flagella of bacteria were damaged or even eliminated, which would cause movement disorders. Many holes or gaps were observed on cell surfaces, which would cause the leakage of cytoplasm and macromolecules, and leading to cell death at last. Our results suggested that silver ions have similar action mode and slightly better antibacterial activity than that of AgNPs against bacterial cells. (C) 2017 Elsevier Ltd. All rights reserved.