Human serum antibodies to varicella-zoster virus thymidine kinase
Human serum antibodies to varicella-zoster virus thymidine kinase
复制标题
DOI：
10.1128/iai.36.1.30-37.1982
复制
发表时间：
1982-04
影响因子：
3.1
通讯作者：
C. Källander;J. Gronowitz;E. Torfason
中科院分区：
文献类型：
--
作者：
C. Källander;J. Gronowitz;E. Torfason
研究了水痘-带状疱疹病毒（VSV）诱导的脱氧胸苷激酶（dTk）产生的条件。发现VZV感染后62 h收获的Vero细胞提取物含有VZV诱导的dTk活性，细胞dTk活性的贡献最小。VZV dTK显示具有广泛的底物特异性，可磷酸化脱氧胸苷、脱氧胞苷和碘脱氧尿苷。脱氧胸苷三磷酸抑制研究显示，与细胞胞质酶和脱氧胸苷三磷酸不敏感的单纯疱疹病毒dTk相比，中间脱氧胸苷三磷酸敏感性。开发了一种VZV dTK阻断抗体的测定法，在存在选择性阻断宿主细胞来源的dTK的脱氧胸苷三磷酸浓度的情况下，以[125 I]碘脱氧尿苷作为底物。共研究了79份血清样本，其中包括水痘或带状疱疹患者的血清对以及免疫和非免疫成人的单一血清。仅在带状疱疹患者血清中检测到VZV dTk阻断抗体。显示VZV dTK血清转化的所有血清对也显示补体结合滴度的平行转化。发现VZV dTk抗体属于免疫球蛋白G类。对VZV dTK的免疫特异性进行了研究，与单纯疱疹病毒1型或2型dTK无交叉反应。
The conditions required for the production of varicella-zoster virus (VSV)-induced deoxythymidine kinase (dTk) have been studied. Extracts from Vero cells harvested 62 h after VZV infection were found to contain VZV-induced dTk activity, with a minimal contribution from the cellular dTk activity. VZV dTK was shown to have a broad substrate specificity phosphorylating both deoxythymidine, deoxycytidine, and iododeoxyuridine. Deoxythymidine triphosphate inhibition studies revealed an intermediate deoxythymidine triphosphate sensitivity when compared with that of the cellular cytosolar enzyme and the deoxythymidine triphosphate-insensitive herpes simplex virus dTk. An assay for VZV dTk-blocking antibodies was developed, with [125I]iododeoxyuridine as a substrate in the presence of a deoxythymidine triphosphate concentration which selectively blocked the dTK of host cell origin. A total of 79 serum samples were studied; these included serum pairs from patients with varicella or herpes zoster and single sera from immune and nonimmune adults. VZV dTk blocking antibodies were detected exclusively in sera from patients with herpes zoster. All serum pairs showing VZV dTK seroconversion also showed a parallel conversion of complement fixation titers. The VZV dTk antibodies were found to be of the immunoglobulin G class. The immunological specificity of VZV dTK was investigated, and no cross-reactivity with herpes simplex virus type 1 or 2 dTk was found.