ARRANGEMENT OF 30S HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN ON POLYOMA-VIRUS LATE NUCLEAR TRANSCRIPTS
ARRANGEMENT OF 30S HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN ON POLYOMA-VIRUS LATE NUCLEAR TRANSCRIPTS
复制标题
DOI：
10.1128/mcb.1.1.21
复制
发表时间：
1981-01-01
影响因子：
5.3
通讯作者：
KAMEN, R
中科院分区：
文献类型：
--
作者：
STEITZ, JA;KAMEN, R
真核细胞核中的异质核[HN] RNA分子与一组明确的丰富，高度保守的蛋白相关联，形成异质性核核糖核蛋白（HNRNP）。这30年代复合物在核转录本上组装的确切方式尚未得到充分证明。为了确定是否可以检测到HNRNP形成的任何位点选择性（例如，对中间序列或预言区域的优先识别）研究了30S HNRNP在特定的核RNA上的分布，研究了多头病毒晚期转录本。杂交研究不仅表明，大多数多瘤晚期核RNA序列可以以30S复合物的形式分离，而且RNP同样位于转录本的中间序列和预言部分。通过RNase T1寡核苷酸指纹分析从天然30S复合物中回收的多瘤病毒特异性RNA证实了后一个结论。在30S级分中对病毒RNA的小片段进行的指纹分析，这些分数幸存于广泛的RNase处理中，表明与中间序列相对应的寡核苷酸优先丢失。这些发现与30S HNRNP的结构及其在RNA生物发生中的功能有关。
Heterogeneous nuclear [hn] RNA molecules in eukaryotic cell nuclei associate with a well-defined group of abundant, highly conserved proteins to form heterogeneous nuclear ribonucleoproteins (hnRNP). The exact manner in which these 30S complexes assemble on nuclear transcripts has not been well documented. To determine whether any site selectivity in the formation of hnRNP can be detected (e.g., preferential recognition of intervening sequences or of premessage regions), the distribution of 30S hnRNP on a particular nuclear RNA, the polyoma virus late transcript, was investigated. Hybridization studies showed not only that the majority of polyoma late nuclear RNA sequences can be isolated in the form of 30S complexes but that the RNP were located equally on intervening sequences and premessage portions of the transcript. The latter conclusion was confirmed by RNase T1 oligonucleotide fingerprint analysis of polyoma virus-specific RNA recovered from native 30S complexes. Fingerprint analysis of the small segments of viral RNA in the 30S fraction that survived extensive RNase treatment revealed that oligonucleotides corresponding to intervening sequences were preferentially lost. These findings are discussed in relation to the structure of 30S hnRNP and their function in RNA biogenesis.