G-ALPHA-16, A G-PROTEIN ALPHA SUBUNIT SPECIFICALLY EXPRESSED IN HEMATOPOIETIC-CELLS
G-ALPHA-16, A G-PROTEIN ALPHA SUBUNIT SPECIFICALLY EXPRESSED IN HEMATOPOIETIC-CELLS
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DOI：
10.1073/pnas.88.13.5587
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发表时间：
1991-07-01
影响因子：
11.1
通讯作者：
SIMON, MI
中科院分区：
文献类型：
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作者：
AMATRUDA, TT;STEELE, DA;SIMON, MI
由鸟嘌呤核苷酸结合调节蛋白（G蛋白）介导的信号转导途径决定了造血细胞的许多反应。最近鉴定的编码G蛋白α-亚基G-α-16的基因在造血谱系的人细胞中特异性表达。G-α-16 cDNA编码的蛋白质的预测M（r）为43，500，其类似于G（q）类α亚基，并且不包括百日咳毒素ADP-核糖基化位点。与其他G蛋白α亚基相比，G-α-16预测蛋白在氨基末端、A区鸟嘌呤核苷酸结合结构域和蛋白羧基末端三分之一处具有独特的氨基酸序列。骨髓单核细胞和T细胞表型的细胞系表达G-α-16基因，但在两个B细胞系或非造血细胞系中检测不到表达。二甲基亚砜诱导HL-60细胞向中性粒细胞分化后G-α-16基因表达下调从对应于G-α-16的两个区域的合成肽产生的抗血清特异性地与过表达G-α-16的细菌菌株和HL-60膜中的42-至43-kDa的蛋白质反应。这种蛋白在二甲基亚砜分化的HL-60细胞膜中减少，在COS细胞膜中检测不到。该基因的限制性表达表明G-α-16调节细胞类型特异性信号转导途径，其不受百日咳毒素抑制。
Signal-transduction pathways mediated by guanine nucleotide-binding regulatory proteins (G proteins) determine many of the responses of hematopoietic cells. A recently identified gene encoding a G protein alpha-subunit, G-alpha-16, is specifically expressed in human cells of the hematopoietic lineage. The G-alpha-16 cDNA encodes a protein with predicted M(r) of 43,500, which resembles the G(q) class of alpha-subunits and does not include a pertussis toxin ADP-ribosylation site. In comparison with other G protein alpha-subunits, the G-alpha-16 predicted protein has distinctive amino acid sequences in the amino terminus, the region A guanine nucleotide-binding domain, and in the carboxyl-terminal third of the protein. Cell lines of myelomonocytic and T-cell phenotype express the G-alpha-16 gene, but no expression is detectable in two B-cell lines or in nonhematopoietic cell lines. G-alpha-16 gene expression is down-regulated in HL-60 cells induced to differentiate to neutrophils with dimethyl sulfoxide. Antisera generated from synthetic peptides that correspond to two regions of G-alpha-16 specifically react with a protein of 42- to 43-kDa in bacterial strains that overexpress G-alpha-16 and in HL-60 membranes. This protein is decreased in membranes from dimethyl sulfoxide-differentiated HL-60 cells and is not detectable in COS cell membranes. The restricted expression of this gene suggests that G-alpha-16 regulates cell-type-specific signal-transduction pathways, which are not inhibited by pertussis toxin.