Detection and molecular characterization of tick-borne pathogens infecting sheep and goats in Blue Nile and West Kordofan states in Sudan
Detection and molecular characterization of tick-borne pathogens infecting sheep and goats in Blue Nile and West Kordofan states in Sudan
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DOI：
10.1016/j.ttbdis.2018.01.014
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发表时间：
2018-03-01
影响因子：
3.2
通讯作者：
Xuan, Xuenan
中科院分区：
文献类型：
--
作者：
Lee, Seung-Hun;Mossaad, Ehab;Xuan, Xuenan
蜱传病原体（tbp）在撒哈拉以南非洲的牲畜中很常见。然而，关于苏丹绵羊和山羊中tbp的信息有限。在这项研究中，使用PCR方法对青尼罗州和西科尔多凡州的178份绵羊和山羊的血液样本进行了tbp调查。总体而言，110份（61.8%）样本被发现感染了至少一种以下病原体：鹅无原体、鹅伊氏杆菌和反刍埃利希菌。未发现巴贝斯虫和莱斯托夸蒂绦虫。最常见的致病菌为羊单胞菌（n=107，占60.1%），其次为羊单胞菌（n=23，占12.9%）和反刍单胞菌（n=1，占0.6%）。绵羊中葡萄球菌和葡萄球菌的患病率明显高于山羊。系统发育分析显示，本研究鉴定的葡萄球菌18S rRNA和葡萄球菌msp4、groEL和16S rRNA聚在一起，表明分子特征相似。此外，通过对E. ruminantium pCS20的系统发育分析，本研究中发现的E. ruminantium属于西非类群，与之前在苏丹蜱中发现的E. ruminantium不同。我们得出的结论是，tbp在研究地区非常普遍，需要对苏丹绵羊和山羊的tbp进行持续监测。
Tick-borne pathogens (TBPs) are common in livestock of sub-Saharan Africa. However, information regarding TBPs in sheep and goats in Sudan is limited. In this study, 178 blood samples of sheep and goats in Blue Nile and West Kordofan states were investigated for TBPs using PCR. Overall, 110 (61.8%) samples were found to be infected with at least one of the following pathogens: Anaplasma ovis, Theileria ovis, and Ehrlichia ruminantium. Babesia ovis and T. lestoquardi were not identified. A. ovis was the most prevalent pathogen (n=107, 60.1%), followed by T. ovis (n=23, 12.9%) and E. ruminantium (n=1, 0.6%). The prevalence rates of A. ovis and T. ovis were significantly higher in sheep than in goats. Phylogenetic analysis of T. ovis 18S rRNA and A. ovis msp4, groEL, and 16S rRNA, revealed that the pathogens identified in this study are clustered together, indicating similar molecular characteristics. Additionally, phylogenetic analysis of E. ruminantium pCS20 revealed that E. ruminantium in this study belong to the West Africa group, and different to E. ruminantium previously identified in ticks from Sudan. We concluded that TBPs are highly prevalent in the study area and continuous monitoring of TBPs in sheep and goats in Sudan is highly required.