Transcriptome and metabolome analyses reveal the pivotal role of hydrogen sulfide in promoting submergence tolerance in Arabidopsis
Transcriptome and metabolome analyses reveal the pivotal role of hydrogen sulfide in promoting submergence tolerance in Arabidopsis
复制标题
转录组和代谢组分析揭示了硫化氢在促进拟南芥耐淹性中的关键作用
DOI：
10.1016/j.envexpbot.2020.104365
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发表时间：
2021
影响因子：
5.7
通讯作者：
Chongying Wang
中科院分区：
文献类型：
--
作者：
Tao Yang;Guoqiang Yuan;Qi Zhang;Lijuan Xuan;Jian Li;Lina Zhou;Huihong Shi;Xinyu Wang;Chongying Wang
随着全球气候变化，洪涝灾害的发生频率和严重程度显著增加，严重影响了农业生产。硫化氢（H2S）作为一种气体信号分子，在植物发育和逆境适应中起着重要作用。先前的研究表明，当动物经历缺氧时，H2S充当氧气传感器。然而，H2S在植物对洪水诱导的低氧胁迫的响应和适应中的作用尚不清楚。在这里，我们报道了外源H2S或Cys预处理显著提高了拟南芥的耐淹性。随着时间的推移，土壤种植的拟南芥的内源H2S及其主要内源产物半胱氨酸（Cys）水平升高，H2S或Cys生物合成或代谢相关基因的表达发生改变，这表明H2S-Cys的稳态可能有助于调节其对洪水的耐受性。转录组学和代谢组学分析表明，外源h2s预处理及时激活了缺氧响应转录因子和缺氧感知相关基因，并通过调节激素信号限制了与生长相关的细胞活动，但对光合作用相关转录物的影响较小。h2s预处理还改变了浸泡过程中代谢物的水平，特别是氨基酸和植物激素的水平。综上所述，本研究为H2S调控作物耐淹低氧胁迫提供了新的思路，并为设计提高作物耐淹性的新策略奠定了基础。
As the global climate changes, the frequency and severity of flood disasters have increased significantly, seriously affecting agricultural production. Hydrogen sulfide (H2S), as a gaseous signal molecule, plays an important role in plant development and stress adaptation. Previous studies suggested that H2S acts as an oxygen sensor when animals experience hypoxia. However, the role of H2S in the response and adaptation to flooding-induced hypoxia stress in plants is poorly understood. Here, we report that pretreatment with exogenous H2S or Cys significantly improved tolerance to submergence in Arabidopsis. Total submergence of soil-grown Arabidopsis increased the levels of endogenous H2S and its main endogenously generated product, cysteine (Cys) over time, and altered the expression of H2S or Cys biosynthesis- or metabolism-related genes, implying that the H2S-Cys homeostasis may contribute to regulation of tolerance to flooding. Transcriptomic and metabolomic analyses demonstrated that exogenous H2S-pretreatment activated hypoxia-responsive transcription factors and hypoxia sensing-related genes in a timely manner and limited cellular activities associated with growth through regulation of hormonal signaling, but had less influence on transcripts associated with photosynthesis. H2S-pretreatment also altered the levels of metabolites, particularly of amino acids and plant hormones, during submergence. Taken together, this study provides new insight into how H2S regulates tolerance to submergence-induced hypoxia stress and lays the foundation to design new strategies for the improvement of submergence resistance in crops.