Evaluating the Adsorbed Water Layer on Polar Stationary Phases for Hydrophilic Interaction Chromatography (HILIC)
Evaluating the Adsorbed Water Layer on Polar Stationary Phases for Hydrophilic Interaction Chromatography (HILIC)
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DOI：
10.3390/separations6020019
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发表时间：
2019-06-01
期刊：
影响因子：
2.6
通讯作者：
Serris, Ioannis
中科院分区：
文献类型：
--
作者：
Guo, Yong;Bhalodia, Nidhi;Serris, Ioannis
固定在极性固定相表面的富含水的液体层对于亲水相互作用色谱 (HILIC) 中极性化合物的保留至关重要。尽管吸附水层的存在已通过多种技术进行了研究和确认，但仍缺乏可轻松确定并与色谱参数关联的定量测量方法。本研究提出了一种简单的测量方法，称为体积比（吸附水层体积与流动相体积的比率），可以使用甲苯洗脱体积轻松确定。使用该方法测量的体积比值表明，在 HILIC 分离常用的固定相中，吸附水层的体积变化范围很大。观察到体积比随着流动相中乙腈含量和乙酸铵浓度的增加而增加。此外，提高柱温还有降低体积比和减少吸附水层的作用。
The water-rich liquid layer immobilized on the surface of the polar stationary phases is critical to the retention of polar compounds in hydrophilic interaction chromatography (HILIC). Although the presence of the adsorbed water layer has been investigated and confirmed by multiple techniques, there is a lack of quantitative measures that can be easily determined and linked to chromatographic parameters. This study proposes a simple measure termed volume ratio (the ratio of the adsorbed water layer volume and the mobile phase volume) that can be easily determined using toluene elution volume. The volume ratio values measured using the proposed method indicate that the volume of the adsorbed water layer varies in a wide range in the stationary phases commonly used in HILIC separation. It was observed that the volume ratio increases with the acetonitrile content and ammonium acetate concentration in the mobile phase. In addition, increasing the column temperature had the effect of reducing the volume ratio and diminishing the adsorbed water layer.