Bald scalp in men with androgenetic alopecia retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells
Bald scalp in men with androgenetic alopecia retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells
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DOI：
10.1172/jci44478
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发表时间：
2011-02-01
影响因子：
15.9
通讯作者：
Cotsarelis, George
中科院分区：
文献类型：
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作者：
Garza, Luis A.;Yang, Chao-Chun;Cotsarelis, George
雄激素性脱发（阿加），也称为普通型秃发，其特征在于毛囊大小显著减小，这可能与毛囊干细胞或祖细胞的损失有关。为了验证这一假设，我们分析了来自阿加个体的秃头和非秃头头皮中毛囊干细胞和祖细胞的存在。通过流式细胞术定量表达细胞角蛋白15（KRT 15）、CD200、CD34和整联蛋白α 6（ITGA 6）的细胞。高水平的KRT15表达与干细胞的小细胞大小和静止特性相关。这些KRT15（hi）干细胞被保存在秃头头皮样本中。然而，CD200（hi）ITGA 6（hi）和CD34（hi）细胞群均具有祖细胞表型，因为它们靠近富含干细胞的隆突区域，但比KRT 15（hi）干细胞更大且更具增殖性，它们显著减少。在功能测定中，来自鼠毛囊的类似CD200（hi）Itga6（hi）细胞是多能的，并且在皮肤重建测定中产生新的毛囊。这些发现支持了毛囊干细胞向祖细胞转化的缺陷在阿加发病机制中起作用的观点。
Androgenetic alopecia (AGA), also known as common baldness, is characterized by a marked decrease in hair follicle size, which could be related to the loss of hair follicle stem or progenitor cells. To test this hypothesis, we analyzed bald and non-bald scalp from AGA individuals for the presence of hair follicle stem and progenitor cells. Cells expressing cytokeratin15 (KRT15), CD200, CD34, and integrin, alpha 6 (ITGA6) were quantitated via flow cytometry. High levels of KRT15 expression correlated with stem cell properties of small cell size and quiescence. These KRT15(hi) stem cells were maintained in bald scalp samples. However, CD200(hi)ITGA6(hi) and CD34(hi) cell populations which both possessed a progenitor phenotype, in that they localized closely to the stem cell-rich bulge area but were larger and more proliferative than the KRT15(hi) stem cells were markedly diminished. In functional assays, analogous CD200(hi)Itga6(hi) cells from murine hair follicles were multipotent and generated new hair follicles in skin reconstitution assays. These findings support the notion that a defect in conversion of hair follicle stem cells to progenitor cells plays a role in the pathogenesis of AGA.