Structure of myosin subfragment 1 from low-angle X-ray scattering.

Structure of myosin subfragment 1 from low-angle X-ray scattering.
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来自低角 X 射线散射的肌球蛋白亚片段 1 的结构。

DOI:
10.1021/bi00558a031
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发表时间:
1980
期刊:
影响因子:
2.9
通讯作者:
Kretzschmar,KM
Kretzschmar,KM
中科院分区:
生物学3区
文献类型:
--
作者:
Mendelson,R;Kretzschmar,KM

文献摘要

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材料与方法蛋白质制备.肌球蛋白亚片段1由不溶性兔背骨骼肌肌球蛋白制备,使用1缩写和符号:SI,肌球蛋白亚片段1; fwhm,半峰全宽; h=(4 tt/X)sin 0; X,X射线波长; 28,散射角; V,分子体积; S,分子表面积; Rg,回转半径; LC 1、LC 2和LC 3,肌球蛋白的轻链,按分子量降序排列; BSA,牛血清白蛋白; NaDodS 04,十二烷基硫酸钠; EDTA,乙二胺四乙酸盐; Tes,7V-三(羟甲基)甲基-2-氨基乙磺酸; AMPPNP,腺苷酰亚氨基二磷酸。
Materials and MethodsProtein Preparation. Myosin subfragment 1 was prepared from insoluble rabbit dorsal skeletal muscle myosin, using1 Abbreviations and symbols used: SI, subfragment 1 of myosin; fwhm, full width at half-maximum; h=(4tt/X) sin 0; X, the X-ray wavelength; 28, the scattering angle; V, the molecular volume; S, the molecular surface area; Rg, the radius of gyration; LC1, LC2, and LC3, the light chains of myosin in descending order of molecular weight; BSA, bovine serum albumin; NaDodS04, sodium dodecyl sulfate; EDTA, ethylenediaminetetraacetate; Tes, 7V-tris (hydroxymethyl) methyl-2-aminoethanesulfonic acid; AMPPNP, adenylylimidodiphosphate.