EMF1, a novel protein involved in the control of shoot architecture and flowering in Arabidopsis
EMF1, a novel protein involved in the control of shoot architecture and flowering in Arabidopsis
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DOI：
10.1105/tpc.13.8.1865
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发表时间：
2001-08-01
期刊：
影响因子：
11.6
通讯作者：
Sung, ZR
中科院分区：
文献类型：
--
作者：
Aubert, D;Chen, LJ;Sung, ZR
被子植物的枝条构型和开花时间取决于大量开花时间和花分生组织特征基因的平衡表达。拟南芥胚花(EMF)基因的功能缺失突变导致拟南芥消除丛生芽的生长，并通过在所有节上产生一朵顶端花来将顶端分生组织从不确定的生长转变为确定的生长。我们已经通过定位克隆的方法鉴定了EMF1基因。该多肽与水稻基因组中的一个已知功能蛋白没有同源性，EMF1与该蛋白具有相同的基序，包括核定位信号、P-loop和LxxL1元件。转基因植株中EMF1表达的改变导致了开花时间、新梢决定力和花序结构的逐渐变化。EMF1及其相关序列可能属于一类新的蛋白质，在新梢发育过程中作为相变的转录调节因子发挥作用。
Shoot architecture and flowering time in angiosperms depend on the balanced expression of a large number of flowering time and flower meristem identity genes. Loss-of-function mutations in the Arabidopsis EMBRYONIC FLOWER (EMF) genes cause Arabidopsis to eliminate rosette shoot growth and transform the apical meristem from indeterminate to determinate growth by producing a single terminal flower on all nodes. We have identified the EMF1 gene by positional cloning. The deduced polypeptide has no homology with any protein of known function except a putative protein in the rice genome with which EMF1 shares common motifs that include nuclear localization signals, P-loop, and LXXLL elements. Alteration of EMF1 expression in transgenic plants caused progressive changes in flowering time, shoot determinacy, and inflorescence architecture. EMF1 and its related sequence may belong to a new class of proteins that function as transcriptional regulators of phase transition during shoot development.