Placental growth throughout the last two thirds of pregnancy in sheep: Vascular development and angiogenic factor expression
Placental growth throughout the last two thirds of pregnancy in sheep: Vascular development and angiogenic factor expression
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DOI：
10.1095/biolreprod.106.054684
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发表时间：
2007-02-01
影响因子：
3.6
通讯作者：
Reynolds, Lawrence P.
中科院分区：
文献类型：
--
作者：
Borowicz, Pawel P.;Arnold, Daniel R.;Reynolds, Lawrence P.
形态计量学方法的发展和应用，以调查在妊娠的最后三分之二母体（carunular； CAR）和胎儿（子叶；COT）羊胎盘的血管发育模式。我们还检测了CAR和COT绵羊胎盘中主要血管生成因子及其受体的表达水平。尽管从妊娠第50天到第140天，CAR组织的血运不断增加，但COT组织的血运增加速度约为CAR瞬时速率（即按比例增加/天）的两倍。对于CAR，从第50天到第140天，血管密度增加了2倍，毛细血管数量增加相对较小，毛细血管直径增加2到3倍。对于COT，血管的增加是由于毛细血管数量增加了12倍，同时毛细血管直径减少了2倍。由于分支增加，胎儿胎盘毛细血管数量大幅增加，导致单位COT组织总毛细血管截面积和总毛细血管表面积增加6倍。在CAR和COT中，血管生成因子及其受体mrna的表达模式不同。CAR的扩张样血管生成与血管内皮生长因子受体-1 （FLT1）、血管生成素-2 （ANGPT2）和可溶性鸟苷酸环化酶（GUCY1B3） mrna的表达相关。COT的支状血管生成与血管内皮生长因子（VEGF）、FLT1、血管生成素-1 （ANGPT1）、ANGPT2、FGF2 mrna的表达相关。监测血管生成因子的表达，并将其水平与血管定量测量相关联，使人们能够以时空方式模拟血管生成。
Morphometric methodologies were developed and applied to investigate the patterns of vascular development in maternal (caruncular; CAR) and fetal (cotyledonary; COT) sheep placentas throughout the last two thirds of gestation. We also examined the expression levels of the major angiogenic factors and their receptors in CAR and COT sheep placentas. Although the vascularity of the CAR tissues increased continuously from Day 50 through Day 140 of pregnancy, those of the COT tissues increased at about twice the instantaneous rate (i.e., the proportionate increase/day) of the CAR. For CAR, vascularity increased 2-fold from Day 50 through Day 140 via relatively small increases in capillary number and 2- to 3-fold increases in capillary diameter. For COT, the increased vascularity resulted from a 12-fold increase in capillary number associated with a concomitant 2-fold decrease in capillary diameter. This large increase in fetal placental capillary number, which was due to increased branching, resulted in 6-fold increases in total capillary cross-sectional area and total capillary surface, per unit of COT tissue. Different patterns of expression of the mRNAs for angiogenic factors and their receptors were observed for CAR and COT. The dilation-like angiogenesis of CAR was correlated with the expression of vascular endothelial growth factor receptor-1 (FLT1), angiopoietin-2 (ANGPT2), and soluble guanylate cyclase (GUCY1B3) mRNAs. The branching-like angiogenesis of COT was correlated with the expression of vascular endothelial growth factor (VEGF), FLT1, angiopoietin-1 (ANGPT1), ANGPT2, and FGF2 mRNAs. Monitoring the expression of angiogenic factors and correlating the levels with quantitative measures of vascularity enable one to model angiogenesis in a spatiotemporal fashion.