Lanthanide-Based Protease Activity Sensors for Time-Resolved Fluorescence Measurements
Lanthanide-Based Protease Activity Sensors for Time-Resolved Fluorescence Measurements
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DOI：
10.1021/ja800322b
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发表时间：
2008-11-05
影响因子：
15
通讯作者：
Kikuchi, Kazuya
中科院分区：
文献类型：
--
作者：
Mizukami, Shin;Tonai, Kazuhiro;Kikuchi, Kazuya
镧系发光传感器值得注意，因为它们的长寿命发光使时间分辨荧光测量成为可能。在探索合适的天线群后，我们设计并合成了基于镧系元素的荧光传感器，用于检测蛋白酶的活性。该传感器在加入蛋白酶如钙蛋白酶I和亮氨酸氨基肽酶（LAP）时产生强发光。由于探针的发光寿命很长，传感器可以应用于时间分辨测量，排除来自蛋白质或其他杂质的背景荧光信号。该传感原理可适用于其他蛋白酶的一般时间分辨荧光测定。
Lanthanide-based luminescent sensors are noteworthy because their long-lifetime luminescence enables time-resolved fluorescence measurements. After exploring suitable antenna groups, we designed and synthesized lanthanide-based luminogenic sensors detecting protease activities. This sensor yielded strong luminescence on addition of proteases such as calpain I and leucine aminopeptidase (LAP). Since the luminescence lifetimes of the probes were very long, the sensors could be applied to time-resolved measurements that exclude background fluorescence signals derived from protiens or other impurities. This sensing principle could be applicable to general time-resolved fluorescence assays for other proteases.