Lateral gene transfer between obligate intracellular bacteria:: Evidence from the Rickettsia massiliae genome
Lateral gene transfer between obligate intracellular bacteria:: Evidence from the Rickettsia massiliae genome
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DOI：
10.1101/gr.6742107
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发表时间：
2007-11-01
期刊：
影响因子：
7
通讯作者：
Raoult, Didier
中科院分区：
文献类型：
--
作者：
Blanc, Guillaume;Ogata, Hiroyuki;Raoult, Didier
立克次体是一种蜱传的专性细胞内α-变形菌，引起人类斑疹热。在这里，我们提出了它的基因组序列，包括一个1.3 Mb的环状染色体和一个15.3 kb的质粒。该染色体与其他斑点热群立克次体基因组具有长程共线性，除了一个大片段特异于R。包含14个tra基因，推测参与菌毛形成和接合DNA转移。我们证明，tra区域最近获得的横向基因转移（LGT）从一个物种有关的立克次体贝利。对基因组序列的进一步分析鉴定了立克次体之间LGT的其他候选者。我们的研究表明，专性细胞内立克次体之间最近的LGT比以前认为的更常见。
Rickettsia massiliae is a tick-borne obligate intracellular alpha-proteobacteria causing spotted fever in humans. Here, we present the sequence of its genome, comprising a 1.3-Mb circular chromosome and a 15.3-kb plasmid. The chromosome exhibits long-range colinearity with the other Spotted Fever Group Rickettsia genomes, except for a large fragment specific to R. massiliae that contains 14 tra genes presumably involved in pilus formation and conjugal DNA transfer. We demonstrate that the tra region was acquired recently by lateral gene transfer (LGT) from a species related to Rickettsia bellii. Further analysis of the genomic sequences identifies additional candidates of LGT between Rickettsia. Our study indicates that recent LGT between obligate intracellular Rickettsia is more common than previously thought.