Characterization of a sensitive biosensor based on an unmodified DNA and gold nanoparticle composite and its application in diquat determination
Characterization of a sensitive biosensor based on an unmodified DNA and gold nanoparticle composite and its application in diquat determination
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DOI：
10.1016/j.arabjc.2015.03.009
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发表时间：
2018-07-01
影响因子：
6
通讯作者：
Kang, Wei Jun
中科院分区：
文献类型：
--
作者：
Niu, Ling Mei;Liu, Ying;Kang, Wei Jun
DNA通常利用DNA分子一端的巯基或氨基来吸附金纳米颗粒。然而，在本文中，我们报告了一种使用具有连续腺嘌呤 (CA DNA) 和金纳米颗粒 (GNP) 的未修饰 DNA 分子构建的灵敏生物传感器。在金电极上制备 CA DNA-GNP 复合材料，并利用扫描电子显微镜 (SEM)、电化学阻抗谱 (EIS) 和电化学方法对其进行表征。使用电化学石英晶体微天平 (EQCM)，还研究了 CA DNA 和 GNP 结合的机制。修饰电极对敌草快表现出超灵敏的响应。采用微分脉冲伏安法(DPV)研究了浓度与还原峰电流之间的线性关系,检测范围为1.0x10(-9)M至1.2x10(-6)M。其检出限为2.0x10(-10)M。考察了该方法在人尿液和谷物中的可行性,得到了满意的结果。 (C) 2015 年作者。
DNA usually adsorbs gold nanoparticles by virtue of mercapto or amino groups at one end of a DNA molecule. However, in this paper, we report a sensitive biosensor constructed using unmodified DNA molecules with consecutive adenines (CA DNA) and gold nanoparticles (GNPs). The CA DNA-GNP composite was fabricated on gold electrodes and characterized by using of scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and the electrochemical method. Using an electrochemical quartz crystal microbalance (EQCM), the mechanism by which the CA DNA and GNPs combined was also studied. The modified electrode exhibited an ultrasensitive response to diquat. Differential pulse voltammetry (DPV) was used to study the linear relationships between concentrations and reduction peak currents, ranging from 1.0x10(-9) M to 1.2x10(-6) M. The detection limit of it is 2.0x10(-10) M. The feasibility of the proposed assay for use in human urine and grain was investigated, and the satisfactory results were obtained. (C) 2015 The Authors.