3D poly (L-lactide)/chitosan micro/nano fibrous scaffolds functionalized with quercetin-polydopamine for enhanced osteogenic and anti-inflammatory activities
3D poly (L-lactide)/chitosan micro/nano fibrous scaffolds functionalized with quercetin-polydopamine for enhanced osteogenic and anti-inflammatory activities
复制标题
槲皮素-聚多巴胺功能化的 3D 聚（L-丙交酯）/壳聚糖微/纳米纤维支架可增强成骨和抗炎活性
DOI：
10.1016/j.cej.2019.123524
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发表时间：
2020-07-01
影响因子：
15.1
通讯作者：
Luo, Binghong
中科院分区：
文献类型：
--
作者：
Zhu, Ling;Chen, Shitian;Luo, Binghong
本文采用3D打印技术和热致相分离技术相结合的方法制备了具有微/纳米纤维分级结构的聚L-乳酸/壳聚糖（PLLA/CS）多孔复合支架。此外，选择生物活性槲皮素（Qu）进一步功能化PLLA/CS支架与聚多巴胺（PDA）层。最终结果表明，所得PLLA/CS-D/Qu支架的亲水性和力学性能明显优于纯3D打印PLLA支架的上级性能。体外MC 3 T3-E1细胞实验表明，CS-100网络的引入有助于细胞向支架内部生长，并促进细胞粘附和增殖。更重要的是，CS-D网络、PDA层和特别是缓释Qu的协同作用，可以赋予PLLA/CS-D/Qu支架不仅具有优异的细胞亲和性和成骨活性，而且具有良好的抗炎性能。因此，PLLA/CS-D/Qu支架材料有望在骨组织工程领域具有良好的应用前景。
We present here a porous poly (L-lactide)/chitosan (PLLA/CS) composite scaffold with micro/nano-fiber hierarchical structure that was innovatively fabricated by combining 3D printing and thermally induced phase separation technology. Moreover, bioactive quercetin (Qu) was chosen to further functionalize the PLLA/CS scaffold with polydopamine (PDA) layer. The final results showed that the hydrophilicity and mechanical properties of the resulting PLLA/CS-D/Qu scaffold were obviously superior to those of the pure 3D printing PLLA scaffold. In vitro MC3T3-E1 cell experiments suggested that the introducing CS nanofiber network helped cells grow into the interior of the scaffold and promoted cell adhesion and proliferation. More importantly, the synergistic effect of the CS nanofiber network, PDA layer and especially sustain-released Qu, can endow the PLLA/CS-D/Qu scaffolds with not only excellent cell affinity and osteogenic activity, but also good anti-inflammatory property. Thus, the PLLA/CS-D/Qu scaffolds are expected to have good application prospects in the field of bone tissue engineering.