Amphotericin B-conjugated biogenic silver nanoparticles as an innovative strategy for fungal infections
Amphotericin B-conjugated biogenic silver nanoparticles as an innovative strategy for fungal infections
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DOI：
10.1016/j.micpath.2016.08.031
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发表时间：
2016-10-01
影响因子：
3.8
通讯作者：
Yuan, Qipeng
中科院分区：
文献类型：
--
作者：
Ahmad, Aftab;Wei, Yun;Yuan, Qipeng
需要新的策略来提高药物的疗效和治疗新出现的微生物耐药性。一种有效的策略是将药物与金属纳米颗粒结合起来控制微生物感染和耐药性。考虑到这一事实，我们开发了一种简单而环保的方案来合成两性霉素b偶联银纳米颗粒及其作为抗真菌剂的评估。利用马氏菌和两性霉素B水提物中的植物化学物质作为封盖剂制备了两种类型的纳米银，即(i)生物源性纳米银（B- agnps）和（ii）两性霉素B偶联生物源性纳米银（Amp-bAgNPs）。利用紫外可见光谱法检测制备的纳米粒子（424 ~ 433 nm）的表面等离子体共振峰（SPR）。高分辨率透射电镜（HRTEM）研究表明，形成了分散良好的球形银纳米粒子和Amp-bAgNPs，平均粒径为10和15 nm。EDX和FTIR研究分别证实了所制备纳米颗粒的元素组成和表面粘附的生物分子。生物源性纳米银对白色念珠菌（16 mm +/- 1.4）和热带念珠菌（18 mm +/- 1.5）的抗真菌活性较低（4-8 mm +/- 0.2），而两性霉素B偶联纳米银对白色念珠菌（16 mm +/- 1.4）和热带念珠菌（18 mm +/- 1.5）具有显著的抗真菌活性。综上所述，Amp-AgNPs偶联体系的抗真菌活性增强是由于两性霉素B的抗真菌活性与银的抗菌性能协同作用所致。本研究结果表明，共轭纳米颗粒可作为有效的抗真菌药物和药物传递载体。此外，这是第一个描述利用马柳草的水萃取物合成纳米银的报告，并将两性霉素B（一种抗真菌药物）偶联到植物合成的纳米银上。(C) 2016 Elsevier Ltd.版权所有。
New strategies are required to improve the efficacy of drugs and to treat the emerging microbial resistance. An effective strategy is to combine drugs with metal nanoparticles for the control of microbial infections and resistance. Keeping in view this fact, we developed a facile and eco-friendly protocol for the synthesis of amphotericin B-conjugated silver nanoparticles and their assessment as an antifungal agent. Phytochemicals from the aqueous extract of Maytenus royleanus and amphotericin B were used as capping agents to prepare two types of silver nanoparticles i.e. (i) biogenic silver nanoparticles (b-AgNPs) and (ii) amphotericin B-conjugated biogenic silver nanoparticles (Amp-bAgNPs). UV-Vis spectroscopy was used to detect the characteristic surface Plasmon resonance peaks (SPR) for the prepared nano particles (424-433 nm). High-resolution transmission electron microscopy (HRTEM) study revealed the formation of well dispersed and spherical silver nanoparticles and Amp-bAgNPs with an average particles size of 10 and 15 nm. EDX and FTIR studies confirmed the elemental composition and surface adhered biomolecules in the prepared nanoparticles respectively. Biogenic silver nanoparticles revealed low to moderate antifungal activity (4-8 mm +/- 0.2), however, the amphotericin B conjugated silver nanoparticles exhibited significant activity against Candida albicans (16 mm +/- 1.4) and Candida tropicalis (18 mm +/- 1.5). In conclusion, the enhanced antifungal activity of the Amp-AgNPs conjugate system is due to the synergy between the antifungal activity of amphotericin B and the antimicrobial property of silver. The findings of this study suggest that the conjugated nanoparticles could be used as efficient antifungal agents and drug delivery vehicles. Furthermore, this is the first report describing the synthesis of silver nanoparticles using the aqueous extract of Maytenus royleanus and the conjugation of amphotericin B, an antifungal drug, to the phytosynthesized silver nanoparticles. (C) 2016 Elsevier Ltd. All rights reserved.