Caffeine-tolerant mutations selected through an at-home yeast experimental evolution teaching lab.
Caffeine-tolerant mutations selected through an at-home yeast experimental evolution teaching lab.
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DOI：
10.1101/2023.01.17.524437
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发表时间：
2023-01-20
期刊：
影响因子：
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通讯作者：
Dunham MJ
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文献类型：
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作者：
Moresi NG;Geck RC;Skophammer R;Godin D;yEvo Students;Taylor MB;Dunham MJ
yEvo是一门针对高中学生的课程，主要围绕着s.s erevisiae的进化实验。为了使课程适应远程教学，我们创建了一个新方案，在咖啡因存在的情况下进化非转基因酵母。进化菌株对咖啡因的耐受性增强，菌落形态明显。许多人拥有拷贝数变异，转座子插入，以及影响已知与咖啡因和TOR信号（被咖啡因抑制）有关的基因的突变，以及其他先前与咖啡因无关的基因。这表明，我们易于使用的居家方案足以让我们对咖啡因耐受性有新的认识。
yEvo is a curriculum for high school students centered around evolution experiments in S. cerevisiae. To adapt the curriculum for remote instruction, we created a new protocol to evolve non-GMO yeast in the presence of caffeine. Evolved strains had increased caffeine tolerance and distinct colony morphologies. Many possessed copy number variations, transposon insertions, and mutations affecting genes with known relationships to caffeine and TOR signaling - which is inhibited by caffeine - and in other genes not previously connected with caffeine. This demonstrates that our accessible, at-home protocol is sufficient to permit novel insights into caffeine tolerance.